血液病及实体瘤T-淋巴细胞亚群的表达

为了研究各种血液病、良性和恶性肿瘤T-淋巴细胞亚群变化关系,及其对诊断、治疗及预后的价值,采用流式细胞术、Cellquest软件及Modfie软件,对1 112例各种血液病、良性和恶性肿瘤的患者进行测定和比较,求出相关性.结果发现,慢性白血病CD3,CD4,CD8和CD4/CD8各项指标极度减低;急性白血病、MDS各项检测指标均较正常人及其它血液病患者明显下降,相互之间有显著差异(P＜0.01);溶血性贫血患者的各项检测指标较正常人及其它各组患者明显增高,尤其CD3,CD4及CD4/CD8比值增高更明显(P＜0.05);特发性血小板减少性紫癜患者CD3,CD4及CD4/CD8比值较正常人明显减低,但与再生障碍性贫血和其它疾病接近,而CD8明显高于正常人及其它血液病、肿瘤患者相互之间有显著差异(P＜0.01);缺铁性贫血、慢性病性贫血、良性肿瘤和其它血液病患者CD3,CD4和CD8虽然均较正常人及溶血性贫血患者有不同程度的减低,但较急性和慢性白血病、恶性肿瘤、粒细胞减少症、MDS患者有不同程度增高,相互之间无显著性差异.值得注意的是,上述各种检测指标异常与治疗前后,以及疾病进展、缓解、预后呈正相关.结论血液病和恶性肿瘤T-细胞亚群各项检测指标对血液病和良、恶性肿瘤诊断及鉴别诊断,以及正确指导治疗及评价预后有重要实用价值,特别对恶性血液病、恶性肿瘤转归有更重要的意义,是目前测定体内免疫活性的最适指标.

Expression of T Cell Markers in Hematological Diseases and Solid Tumor

To explore the relationship between T cell markers in hematological diseases and T cell markers in solid tumor, CD3, CD4, CD8 in hematological diseases, malignant and benign tumors were detected by flow cytometry and results were analyzed statistically. The results showed that CD3, CD4, CD8 and CD4/CD8 in chronic leukemia decreased significantly while these markers in acute leukemia and MDS decreased obviously in comparison with normal persons and other hematological diseases (P < 0.0l). Hemolytic anemia markers increased significantly (P < 0.05). CD3, CD4, CD4/CD8 in idiopathic thrombocytopenic purpura decreased and CD8 increased (P < 0.0l). CD3, CD4, CD8 in iron-deficiency anemia, anemia from chronic diseases, benign tumor and other hematological diseases were lower than those in normal persons and hemolytic anemia, but higher than those in acute and chronic leukemia, malignant tumor, granulocytopenia, and MDS (P > 0.05). It is notable that the above markers correlated with the development and prognosis of diseases. In conclusion, expression of CD3, CD4, CD8, CD4/CD8 contributes to diagnosis of hematological diseases and benign or malignant tumors, and is an important indicator for therapeutic strategy.