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| 弓形虫STAg不同途径免疫小鼠诱导的黏膜免疫和系统免疫应答动态变化 | |
| 引用本文： | 刘美娜,殷国荣,孟晓丽,殷丽天,申金雁,刘红丽. 弓形虫STAg不同途径免疫小鼠诱导的黏膜免疫和系统免疫应答动态变化[J]. 中国病原生物学杂志, 2010, 0(4) |
| 作者姓名： | 刘美娜 殷国荣 孟晓丽 殷丽天 申金雁 刘红丽 |
| 作者单位： | 山西医科大学医学寄生虫学研究所寄生虫学教研室;山西医科大学生理学系细胞生理学省部共建教育部重点实验室; |
| 基金项目： | 国家自然科学基金项目(No.30640057); 山西医科大学青年基金项目(No.02200713) |
| 摘 要： | 目的动态观察弓形虫可溶性速殖子抗原(soluble tachyzoite antigen,STAg)滴鼻和皮下免疫小鼠诱导的黏膜免疫和系统免疫应答。方法6周龄BALB/c小鼠90只随机分为3组,分别以20μg STAg滴鼻或皮下注射免疫小鼠2次,间隔2周,对照组不做处理。末次免疫后1、2、3、4和5周,每组随机处死6只小鼠。ELISA法测定小肠冲洗液sI-gA和血清IgG,分离并计数小肠上皮内淋巴细胞(intraepithelial lymphocyte,IEL)和脾淋巴细胞。结果对照组小鼠小肠冲洗液sIgA水平和IEL数及血清IgG水平和脾淋巴细胞数均维持在较低水平。滴鼻免疫组小鼠小肠冲洗液sIgA水平升高,与对照组比较差异有统计学意义(FsIgA=10.074,FIEL=14.747,P0.01),其中第5周sIgA水平、第2~5周IEL数量与皮下免疫组比较差异有统计学意义(FsIgA=7.862,FIEL=9.807,P0.05)。皮下免疫组小鼠血清IgG水平和脾淋巴细胞数均升高,与对照组比较差异有统计学意义(FIgG=8.207,F脾细胞=11.209,P0.05),第5周脾淋巴细胞数与滴鼻组比较差异有统计学意义(F=6.826,P0.05)。结论20μg STAg滴鼻免疫小鼠诱导的黏膜免疫应答水平优于同剂量的皮下免疫,同时也诱导了较高水平的系统免疫应答。皮下免疫可诱导较高水平的系统免疫应答,但其诱导的黏膜免疫应答较弱。 |
| 关 键 词： | 刚地弓形虫 可溶性速殖子抗原 鼻内免疫 黏膜免疫 免疫途径 |
Kinetics of mucosal and systemic immune responses of mice vaccinated with Toxoplasma gondii soluble tachyzoite antigen via various routes |
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| LIU Mei-na,YIN Guo-rong,MENG Xiao-li,YIN Li-tian,SHEN Jin-yan,LIU Hong-li. Kinetics of mucosal and systemic immune responses of mice vaccinated with Toxoplasma gondii soluble tachyzoite antigen via various routes[J]. Journal of Pathogen Biology, 2010, 0(4) | |
| Authors: | LIU Mei-na YIN Guo-rong MENG Xiao-li YIN Li-tian SHEN Jin-yan LIU Hong-li |
| Affiliation: | LIU Mei-na1,YIN Guo-rong1,MENG Xiao-li1,YIN Li-tian,SHEN Jin-yan,LIU Hong-li(1.Institute of Medical Parasitology,Shanxi Medical University,Taiyuan 030001,China,2.Department of Physiology,Key Laboratory of Cellular Physiology Co-constructed by Province , Ministry of Education,Shanxi Medical University) |
| Abstract: | Objective To investigate the kinetics of mucosal immune and systemic immune responses induced by intranasal or subcutaneous immunization of mice with Toxoplasma gondii-soluble tachyzoite antigen(STAg).Methods Ninety 6-week-old BALB/c mice were randomly divided into three groups.Mice in the intranasal and subcutaneous injection group were respectively immunized two times with 20 g STAg,at an interval of 2 weeks,while the control group was untreated.Six mice from each group were sacrificed respectively at wee... |
| Keywords: | Toxoplasma gondii STAg intranasal immunization mucosal immunity immune route |
| 本文献已被 CNKI 等数据库收录！ | |