泻火解毒、消瘀除痰法对脑出血大鼠钙及细胞间黏附分子-1的影响

目的探讨泻火解毒、消瘀除痰法对脑出血（ICH）大鼠钙（[Ca^2＋]i）、细胞间黏附分子-1（ICAM-1）的作用及可能机制。方法将成年SD大鼠随机分为6组：正常对照组、手术对照组、模型对照组、脑清汤（NQT）大、小剂量组、清开灵注射液（QKL）对照组。胶原酶注入右侧尾壳核ICH造模，荧光示踪法检测对ICH大鼠脑细胞[Ca^2＋]i的影响，免疫组织细胞化学技术观察在ICH不同时段对ICAM-1表达的影响。结果NQT大、小剂量组及QKL对照组能显著拮抗脑细胞[Ca^2＋]i升高（P〈0．01），显著抑制ICAM-1表达，并使其表达持续时间缩短。结论NQT可阻止钙超载的发生，精密调节细胞因子网络、阻滞细胞因子和炎症介质释放而抗炎，从而保护神经元。

Effect of TCM Treatment for Purging Fire, Removing Toxic Substance, Relieving Stagnation and Eliminating Phlegm on Cytoplasmic Free Calcium and Intercellular Adhesion Molecule-1 in Rats with Intracerebral Hemorrhage

Objective To study the effects and possible mechanisms of TCM treatment for purging fire, removing toxic substance, relieving stagnation and eliminating phlegm on cytoplasmic free calcium (Ca2 i) and intercellular adhesion molecule-1 (ICAM-1) in rats with intracerebral hemorrhage (ICH). Methods Adult SD rats were randomly divided into six groups: the normal group, the operated group, the model group, the Naoqing Decoction (NQD) low- and high-dose groups, and the Qingkailing Injection (QKL) group. ICH model was induced by injecting collagenase into right caudate nucleus, level of Ca2 i was determined by fluorescent tracing assay, and the expression of ICAM-1 was determined using immunohistochemical technique. Results High- and low-dose of NQD and QKL could significantly antagonize the collagenase injection induced increase of Ca2 i (P<0.01), inhibit the expression of ICAM-1 and shorten its sustainable time. Conclusion NQD plays a role in protecting neuron by inhibiting calcium overload, regulating cytokines network, and inhibiting inflammatory reaction through postponing the release of cytokines and inflammatory mediators.