| gp120mAb对gp120抑制大鼠海马脑片CA1区LTP作用的研究 |
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| 引用本文： | 董军,江振友. gp120mAb对gp120抑制大鼠海马脑片CA1区LTP作用的研究[J]. 中国免疫学杂志, 2005, 21(5): 355-358 |
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| 作者姓名： | 董军 江振友 |
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| 作者单位： | 1. 暨南大学医学院病理生理教研室,广州,510632
2. 暨南大学医学院微生物和免疫学教研室,广州,510632 |
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| 基金项目： | 本课题受广东省医学科研基金项目(A2004327),广东省自然科学基金项目(04010443),广东省中医药管理局项目(1040074)资助 |
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| 摘 要： | 目的探讨人类免疫缺陷病毒Ⅰ型(HIV-1)的包膜糖蛋白gp120特异抗体gp120mAb对gp120引起大鼠海马脑片CA1区的突触传递及可塑性变化的影响.方法应用离体脑片记录技术,记录大鼠海马CA1区的兴奋性突触后电位(EPSP),研究gp120mAb对gp120抑制高频电刺激Schaffer侧支引起的鼠海马长时程增强效应(LTP)作用的影响.结果gp120对高频电刺激(HFS,100 Hz,1 000 ms×2,串间隔20秒,共2次)Schaffer侧支引起的大鼠海马CA1区LTP产生抑制作用,而对其基础EPSP没有影响.用浓度为200 pmol/L的gp120灌流脑片,可引起LTP的维持发生抑制.这种抑制作用可被gp120特异抗体gp120mAb(50 ng/ml)所拮抗.结论gp120mAb可能是通过拮抗gp120抑制海马CA1区的LTP诱发和维持而参与艾滋病痴呆(HIV-1 associated dementia,HAD)的形成.
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| 关 键 词： | 人类免疫缺陷病毒Ⅰ型 gp120特异抗体 长时程增强 海马脑片 |
| 文章编号： | 1000-484X(2005)05-0355-04 |
Effect of gp120mAb on long-term potentiation inhibited by HIV-1 gp120 in rat hippocampus in vitro |
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| DONG Jun,JIANG Zhen-you. Effect of gp120mAb on long-term potentiation inhibited by HIV-1 gp120 in rat hippocampus in vitro[J]. Chinese Journal of Immunology, 2005, 21(5): 355-358 |
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| Authors: | DONG Jun JIANG Zhen-you |
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| Affiliation: | DONG Jun,JIANG Zhen-You.Department of Pathophysiology,Medical College of Jinan University,Guangzhou 510632,China |
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| Abstract: | Objective:To explor the effect of gp120mAb on synaptic transmission and plasticity changed by gp120 in the CA 1 region of rat hippocampal slices.Methods:We recorded the excitatory postsynaptic potentials (EPSP) and investigated effect of gp120mAb on long-term potentiation inhibited by gp120 in rat hippocampus in vitro.Results:The results showed that HIV-1 gp120 can inhibit the LTP induced by high-frequence stimulation schaffer in CA 1,gp120 had no effect on basal synaptic transmission.HIV-1 gp120(200 pmol/L) can inhibit the LTP maintance,gp120mAb of gp120-specific antibodies can reverse the inhibition effect,But IgG can't reverse it.Conclusion:The reverse effect of gp120-specific antibody gp120mAb on long-term potentiation inhibited by HIV-1 gp120 may contribute to the HAD pathogenesis. |
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| Keywords: | HIV-1 gp120mAb Long-term potentiation Hippocampal slices |
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