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| 恶性疟原虫多表位蛋白疫苗M.RCAg-1和M.RCAg-3佐剂的配伍 | |
| 引用本文： | 马瑞森,蔺亚晖,张文文,麻明,王恒. 恶性疟原虫多表位蛋白疫苗M.RCAg-1和M.RCAg-3佐剂的配伍[J]. 基础医学与临床, 2011, 31(5) |
| 作者姓名： | 马瑞森 蔺亚晖 张文文 麻明 王恒 |
| 作者单位： | 中国医学科学院,基础医学研究所,北京协和医学院,基础学院,病原生物学系,北京,100005 |
| 基金项目： | 国家高技术研究发展(863)计划 |
| 摘 要： | 目的 评价恶性疟原虫多表位蛋白疫苗M.RCAg-1和M.RCAg-3与不同佐剂最优配伍方式并进行初步的免疫机制研究.方法 将多表位疫苗表达纯化后混合4种佐剂(AD、ADL、CpG及CpG&A1)经皮下注射BALB/c小鼠,免疫3次,每次间隔2周.测定血清中抗M.RcAg-1和M.RCAg-3抗体滴度、IgG分型及血清抗体对抗原单表位识别.间接免疫荧光检测血清对天然疟原虫的识别.结果 佐剂ADL和AD分别能显著增强M.RCAg-1(P<0.05)和M.RCAg-3(P<0.05)免疫反应.而佐剂CpG和CpG&A1增强抗原免疫反应的能力相对较弱.与M.RCAg-1、M.RCAg-3和CpG、CpG&A1配伍的组相比,AD和ADL佐剂组的抗体水平和间接免疫荧光反应也都显著增强(P<0.05,P<0.05),抗体对单表位识别显著,抗体IgG分型中IgG1和IgG2a水平较高.结论 佐剂ADL和AD的效果显著,可以作为与M.RCAg-1和M.RCAg-3配伍的候选临床应用佐剂. |
| 关 键 词： | 恶性疟原虫 多表位疫苗 佐剂配伍 |
Effects and immune mechanism of different adjuvants on polyepitope chimeric vaccine M. RCAg-1 & M. RCAg-3 |
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| MA Rui-sen,LIN Ya-hui,ZHANG Wen-wen,MA Ming,WANG Heng. Effects and immune mechanism of different adjuvants on polyepitope chimeric vaccine M. RCAg-1 & M. RCAg-3[J]. Basic Medical Sciences and Clinics, 2011, 31(5) | |
| Authors: | MA Rui-sen LIN Ya-hui ZHANG Wen-wen MA Ming WANG Heng |
| Affiliation: | MA Rui-sen,LIN Ya-hui,ZHANG Wen-wen,MA Ming,WANG Heng(Dept.of Pathogen Biology,School of Basic of Medicine,IBMS,CAMS & PUMC,Beijing 100005,China) |
| Abstract: | Objective To investigate the formulations of Plasmodium falciparum polyepitope chimeric vaccine M.RCAg-1 & M.RCAg-3 with different adjuvants in order to increase their immunogenicities and to find out their basic immune mechanisms.Methods The polyepitope chimeric vaccines formulated with different adjuvants(AD,ADL,CpG,CpG&Al) were subcutaneously injected into mice for three times.The immunization was performed on week 0,2,4.The concentration of anti-M.RCAg-1 and anti-M.RCAg-3 IgG,the IgG isotype and the rec... |
| Keywords: | Plasmodium falciparum polyepitope chimeric vaccine adjuvants formulation |
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