新疆药用阿魏属植物内生真菌的生态分布与多样性

为了探索新疆药用阿魏属植物内生真菌的生态分布与多样性,采用微生物学研究方法与技术,从多伞阿魏、阜康阿魏、大果阿魏、新疆阿魏、里海阿魏及羊食阿魏的根、叶及茎中分离内生真菌;运用形态和分子生物学方法对其进行了鉴定;并使用Shannon-Wiener 多样性指数和Sorenson 系数,分析6种药用阿魏内生真菌的组成、分布、多样性及相似性.分离得到内生真菌337株,分属于38个属,其中,链格孢属、短梗霉属及镰刀菌属为优势菌群;在新疆阿魏中分离得到的内生真菌最多,阜康阿魏根中分离出来的内生真菌Shannon-Wiener多样性指数(H')最大,达到1.85;多伞阿魏叶和新疆阿魏叶的内生真菌菌群相似性最高,相似性系数达到0.75.结果显示,6种新疆药用阿魏植物中皆存在内生真菌;阿魏不同部位内生真菌的分布、多样性及相似性等均存在差异,具有一定的物种及组织专一性;为阿魏属植物内生真菌的深入研究奠定了理论基础.

Ecological distribution and diversity of medical Ferula species produced in Xinjiang

To study the ecological distribution and diversity of endophytic fungi associated with Ferula of medicinal plants in Xinjiang.The endophytic fungi were isolated from roots, stems and leaves of Ferula by microbiology research methods and technology.The endophytic fungi were identified using ITS rDNA sequence analysis and morphology analysis.The composition, diversity and preference of endophytic fungal community were analyzed with Shannon-Wiener biodiversity index (H') and Sorensen coefficient (Cs).A total of 337 strains endophytic fungi were isolated and classified into 38 genera, Alternaria, Aureobasidium and Fusarium were the dominant genera.Among the 337 isolates, the endophytic fungi of F.sinkiangensis were the most, The Shannon-Wiener biodiversity index(H') associated with roots of F.fukanensis was the highest, reached 1.85.The highest Sorensen coefficient (Cs) was between leaf of F.sinkiangensis and leaf of F.ovina, reached 0.75.From the result, endophytic fungi were widely distributed in six Ferula, there are some notable differences between distribution and composition of the endophytic fungi isolated from different issues and different species of Ferula, show a certain degree of species and tissue preference.The results obtained in this study will provide realistic basis and theoretical basis for further study the secondary metabolites of endophytic fungi associated with Ferula, and the relationship between endophytic fungi and their host plants.