抗感染组织工程化骨的生物特性

目的探索抗感染组织工程化骨一期修复感染性骨折、骨缺损的新途径。方法昆明小鼠84只,雄性,体重24～27g。其中30只于右侧股部肌袋植入利福平组织工程化骨做药物释放特性观察。54只以随机数字法分为A,B,C3组,每组18只。将植骨材料植入小鼠右侧股部肌袋中。A组植入利福平组织工程化骨,B组植入单纯组织工程化骨,C组植入牛松质骨,做成骨活性观察。结果①此种材料21d内骨粒周围软组织的利福平浓度(1.8mg/L)高于金黄色葡萄球菌的最小抑菌浓度(0.008～0.06mg/L),而小鼠血药浓度很低。②此种材料的成骨活性与单纯组织工程化骨无明显差别(P>0.05),均较单纯牛松质骨载体的成骨活性好(P<0.01)。碱性磷酸酶(μmol/s·kg)测定显示:7dA组43±12,B组48±10,C组22±5,F=7.56,P<0.01;14dA组65.3±3.2,B组63.0±2.8,C组18.2±2.7,F=34.86,P<0.01;28dA组122±20,B组133±12,C组27±7,F=53.86,P<0.01。结论一定剂量的利福平组织工程化骨有较好的成骨活性和极好的抗感染能力,且能保持很低的血药浓度。

Bioactivity of anti- infectious tissue- engineered bone

Aim To explore new way of anti- infectious tissue- engineered bone during primary recovery in treating infectious fracture and bony defects. Methods Eighty- four male Kunming mice weighted from 24 to 27 g were prepared.The muscle bags inside right thigh of thirty mice were implanted rifampin tissue- engineered bone for observing characteristics of drug release in vivo.Another fifty- four mice were divided randomly into A,B and C group according to random number method(eighteen mice in each group).The mice in A group were implanted with rifampin tissue- engineered bone,those in B group were implanted with simple tissue- engineered bone and those in C group were implanted with bovine spongy bone.Then the osteoinductive ability of those were observed.Results ① The rifampin concentration(1.8 mg/L) in surrounding soft tissue of bone granulation was higher than minimal isorrheic concentration(MIC) of staphylococcus aureus(0.008 to 0.06 mg/L) within 21 days after using this material and the blood drug concentration of mice was lower.② There was no significant difference in the osteoproductive ability between this material and simple tissue- engineered bone(P >0.05).Which all had better effects than that of simple bovine spongy carrier(P< 0.001).Measurement of alkaline phosphatease(μ mol/s@ kg) showed that it was 43± 12 in A group, 48± 10 in B group and 22± 5 in C group(F=7.56,P< 0.01) on 7th day and 65.3± 3.2 in A group, 63.0± 2.8 in B group and 18.2± 2.7 in C group(F=34.86,P< 0.01) on 14th day,and 122± 20 in A group, 133± 12 in B group and 27± 7 in C group(F=53.86,P< 0.01) on 28th day.Conclusion Anti- infectious tissue- engineered bone with some dose has high osteoinductive ability and anti- infectious activity with little blood drug concentration.