TP对脂多糖介导人牙周膜成纤维细胞细胞间黏附分子-1表达的影响

目的: 检测人牙周膜成纤维细胞(human periodontal ligament fibroblasts,HPDLF)在脂多糖(lipopolysaccharide,LPS)介导下细胞间黏附分子-1﹙intercellular adhesion molecule-1,ICAM-1﹚的表达,探讨TP(tea polyphenols,TP)对其表达的影响。方法: HPDLF采用改良组织块法体外培养,将HPDLF随机分成LPS组、TP100组、TP200组,用酶联免疫吸附试验法(enzyme linked immuosorbent,ELISA)检测不同浓度TP对LPS介导下HPDLF分泌ICAM-1的活性。实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,Real-time PCR)检测ICAM-1 mRNA的表达。结果: 在LPS干预下,不同时间不同浓度的TP影响下均可抑制ICAM-1分泌,其活性降低,与LPS组比较差异有统计学意义(P<0.05);且ICAM-1 mRNA表达水平显著降低,与LPS组比较差异有统计学意义(P<0.05)。结论: TP对ICAM-1的抑制作用明显,100 μg/mL TP对其的抑制作用更显著,提示TP的抗炎机制可能与抑制ICAM-1有关。

Effect of Tea Polyphenols on ICAM-1 Expression in LPS-mediated Human Periodontal Ligament Fibroblast Cells.

Objective: To investigate the effect of tea polyphenols (TP) on ICAM-1 expression in LPS-treated human periodontal ligament fibroblast cells (HPDLFs). Methods: HPDLFs were obtained from periodontal tissues by modified-cultured method. After induced by LPS, the control group was only treated with LPS, the experiment groups were treated with 100 μg/mL TP (TP100 group) or 200 μg/mL TP (TP200 group). The levels of ICAM-1 secretion in three groups were tested by ELISA. And the mRNA expression of ICAM-1 was detected by real-time PCR. Results: ICAM-1 was inhibited by TP after induced by LPS, and its activity was decreased significantly (P<0.05). The ICAM-1 mRNA levels of TP treated groups were significantly lower than that of LPS group (P<0.05). Conclusion: TP inhibited the expression of ICAM-1 and the effect was more significantly at 100 μg/mL. This result suggested that the anti-inflammatory mechanism of TP might relate to the inhibition of ICAM-1.