莪术醇对人卵巢癌SKOV3细胞增殖、迁移、侵袭及凋亡的影响

目的：探讨莪术醇对人卵巢癌SKOV3细胞增殖、迁移、侵袭及凋亡的影响。方法：采用CCK-8法检测不同浓度（5、10、25、50、100 μmol/L）莪术醇，0 μmol/L莪术醇为对照组，干预SKOV3细胞24 h、48 h的增殖抑制率，并计算IC50值；划痕实验和Transwell小室法分别检测细胞的迁移和侵袭能力，流式细胞术检测细胞凋亡率；Western blot法检测莪术醇及PI3K/AKT通路阻断剂LY294002对细胞PI3K、p-PI3K、AKT、p-AKT蛋白表达的影响。结果：莪术醇对SKOV3细胞的增殖有明显抑制作用，呈浓度依赖性但不呈现时间依赖性，IC50值为48 μmol/L；莪术醇能明显抑制SKOV3细胞的迁移与侵袭，促进其凋亡（均P＜0.01），降低细胞PI3K、AKT总蛋白表达（均P＜0.05），显著降低其活化形式p-PI3K、p-AKT的表达（均P＜0.01）；莪术醇与LY294002联用后表现出良好的协同作用，较单独用莪术醇时下调p-PI3K、p-AKT蛋白更为显著（P＜0.05）。结论：莪术醇能抑制SKOV3细胞增殖、迁移及侵袭，并诱导其凋亡，其作用机制与抑制PI3K/AKT通路有关。

Effects of curcumol on proliferation,migration, invasion and apoptosis of human ovarian cancer SKOV3 cells

Objective: To investigate the effects of curcumol on proliferation, migration, invasion and apoptosis of human ovarian cancer SKOV3 cells. Methods: The CCK-8 method was used to detect the inhibition rate of SKOV3 cells at different concentrations (0, 5, 10, 25, 50, 100 μmol/L) for 24 h and 48 h, and the IC50 value was calculated. The scratch test and Transwell chamber method were used to detect the cells. Migration and invasion ability, apoptosis rate was detected by flow cytometry, Western blot was used to detect the effects of curcumol and PI3K/AKT pathway blocker LY294002 on the expression of PI3K, p-PI3K, AKT and p-AKT proteins. Results: Curcumol significantly inhibited the proliferation of SKOV3 cells in a concentration-dependent but time-independent manner, with an IC50 value of 48 μmol/L. Meanwhile, curcumol significantly inhibited the migration and invasion of SKOV3 cells and promoted apoptosis (P<0.01). The expression of total protein PI3K and AKT was decreased (P<0.05), and the expression of phosphorylated forms p-PI3K and p-AKT was significantly decreased (P<0.01). The combination of curcumol and LY294002 showed a good synergistic effect, which was more obvious than that of the curcumol alone in the down-regulation of p-PI3K, p-AKT (P<0.05). Conclusion: Curcumol can inhibit the proliferation, migration and invasion of SKOV3 cells and induce apoptosis, which is related to the inhibition of PI3K/AKT pathway.