老年肌少症患者外周血白细胞及淋巴细胞亚群的分析

目的：测定肌少症患者外周血白细胞、淋巴细胞亚群、C-反应蛋白（CRP），并探讨其在肌少症发病机制中的作用。方法：选取2017年6月至2018年2月温州市中心医院就诊的120例老年患者，其中肌少症患者48例，非肌少症72例，分别检测外周血白细胞、淋巴细胞亚群和CRP。结果：肌少症组外周血白细胞计数为（6.7±0.9）×109/L，非肌少症组为（5.5±0.8）×109/L；肌少症组血NK细胞（CD3-CD56+）为（30.4±6.6）%，非肌少症组为（25.4±7.2）%；肌少症组血CRP为（6.3±1.2）mg/L，非肌少症组为（3.8±0.6）mg/L，差异均有统计学意义（P＜0.05）。而2组间外周血总淋巴细胞、CD4+T细胞、CD8+T细胞、B细胞、NK样细胞（CD3+CD56+）、记忆T细胞（CD4+CD45RO+）、初始T细胞（CD4+CD45RA+）、双阴T淋巴细胞（CD3+CD4-CD8-）水平的差异均无统计学意义（P＞0.05）。结论：肌少症患者组外周血中白细胞计数、NK细胞、CRP均高于非肌少症组，提示肌少症组患者可能存在机体微炎症和固有免疫反应过强。

Clinical study of leukocyte and lymphocyte subsets of peripheral blood in old sarcopenia patients

Objective: To explore preliminarily the role of immune cells in pathogenesis of sarcopenia by detecting leukocyte, lymphocyte subsets and CRP of peripheral blood in patients with sarcopenia. Methods: A total of 120 old patients including 48 patients with sarcopenia and 72 patients without sarcopenia were selected in our study for the detection of leukocyte, lymphocyte subsets and CRP. Results: Leukocyte count of peripheral blood was (6.7±0.9)×109/L in sarcopenia group, and (5.5±0.8)×109/L in control group; NK cell was (30.4±6.6)% in sarcopenia group, and (25.4±7.2)% in control group; CRP was (6.3±1.2) mg/L in sarcopenia group, and (3.8±0.6) mg/L in control group. There were statistical differences between sarcopenia group and control group. However, the levels of total lymphocytes, CD4+T cells, CD8+T cells, B cells, NK like cells (CD3+CD56+), memory T cells (CD4+CD45RO+), naive T cells (CD4+CD45RA+), double negative T cells (CD3+CD4-CD8-) were not significantly different between sarcopenia group and control group. Conclusion: Leukocyte count, NK cells and CRP of sarcopenia patients are higher than the controls, which indicates micro-inflammatory and hypersensitivity of innate immunity in sarcopenia patients.