姜黄素类似物L6H3对帕金森病大鼠脑部纹状体中单胺类神经递质代谢物的影响

目的：研究姜黄素类似物L6H3对帕金森病（PD）大鼠脑纹状体中7种单胺类神经递质及其代谢物的影响。方法：采用脑部纹状体定位注射6-羟基多巴胺（6-OHDA）建立PD大鼠模型，将大鼠随机分为假手术组、PD组和PD+L6H3组。通过微透析活体取样技术和高效液相色谱电化学检测法（HPLC-ECD）联用测定PD组大鼠脑纹状体细胞外液中单胺类神经递质及其代谢物含量。结果：造模后第6周（即给药第2周）及造模后第7周（即停药第1周），PD+L6H3组大鼠脑纹状体的多巴胺（DA）、3，4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）、去甲肾上腺素（NE）、肾上腺素（E）、5-羟色胺（5-HT）、5-羟基吲哚乙酸（5-HIAA）与PD组比较明显升高（P＜0.01）。结论：L6H3在给药后第2周及停药后1周均能明显提高PD大鼠脑中单胺类神经递质及其代谢物的含量。

The effect of curcumin analogue L6H3 on striatum monoamine neurotransmitter in the rat model of Parkinson disease

Objective: To investigate the effect of curcumin analogue L6H3 on seven monoamine neurotransmitters and metabolite in the striatum of rats with Parkinson disease (PD) by micro-dialysis sampling coupled with high performance liquid chromatography and electrochemical detection (HPLC-ECD). Methods: PD model was established by injecting 6-OHDA into the right striatum of rats, then verified by the rotational behavior after intraperitoneal injection of apomorphine solution. Rats were randomly divided to Sham group, PD group and PD+L6H3 group. The microdialysis sampling coupled HPLC-ECD was developed and applied to detect the level of monoamine neurotransmitters and metabolite in rat striatum. Results: 6 weeks (2 weeks after administration),7 weeks (1 week after stopping administration) after model establishment, the levels of DA, DOPAC, HVA, NE, E, 5-HT, 5-HTAA in PD+L6H3 group were significantly increased compared with PD group (P<0.01). Conclusion: L6H3 has the capability to up-regulate monoamine neurotransmitters and metabolite in the striatum of PD rat model at both 2 weeks after administration and 1 week after stopping administration.