PRRX1的表达对卵巢癌血管生成拟态形成及患者预后的影响

目的:检测配对相关同源框1(PRRX1)及其通路分子在卵巢癌中表达情况以及其与血管生成拟态(VM)的关系，推测PRRX1对卵巢癌患者预后的影响。方法:采用免疫组化法检测62例卵巢癌中 PRRX1、Wnt5a、β-catenin的表达情况，采用CD31/PAS双染法检测卵巢癌中VM的情况。采用χ²检验和Pearson相关分析来分析PRRX1与VM形成和临床病理资料之间的关系以及PRRX1与Wnt5a、β-catenin的关系。采用Kaplan-Meier法评估 PRRX1对卵巢癌患者生存预后的影响。结果:PRRX1表达对卵巢癌患者的FIGO分期、转移及VM形成有影响且具有统计学意义(P <0.05)，而对患者的年龄、肿瘤大小、组织学类型、肿瘤分化程度的影响无明显的统计学差异(P >0.05)。PRRX1表达与β-catenin的核表达有关，与Wnt5a、β-catenin的浆表达无关。PRRX1阳性表达和有VM形成的患者生存时间比PRRX1阴性表达或无VM形成的患者短(P <0.05)且预后差；PRRX1阳性表达和β-catenin核阳性表达的患者生存时间短于PRRX1阴性表达或β-catenin核阴性表达的患者(P <0.05)；而PRRX1阳性表达与Wnt5a阳性表达、β-catenin的浆阳性表达的患者生存时间与PRRX1阴性表达或Wnt5a阴性表达、β-catenin的浆阴性表达的患者生存时间相比，差别无意义(P >0.05)。结论:PRRX1通过 Wnt/β-catenin信号通路调节VM形成从而影响卵巢癌患者预后，PRRX1可作为卵巢癌诊断和预后预测的指标。

Effect of PRRX1 expression on VM formation and prognosis in ovarian cancer

Objective: To investigate the paired related homeobox 1 (PRRX1) and its pathway molecules expression in ovarian cancer and its relationship with the vasculogenic mimicry(VM) formation, so as to explore PRRX1 effect on metastasis and prognosis in ovarian cancer. Methods: The expressions of PRRX1,Wnt5a,β-catenin as well as VM in ovarian cancer samples from 62 cases were examined by immunohistochemical and CD31/periodic acid-Schiff double staining. And the relationships between PRRX1 and VM formation, Wnt5a,β-catenin were assessed byχ² and pearson tests. The prognostic values of PRRX1 were measured by Kaplan-Meier survival analysis. Results: PRRX1 expression was associated with FIGO stage, metastasis and VM formation(P <0.05).No significant correlations were found between PRRX1 and age, tumor size, histological type, degree of differentiation of the tumor(P >0.05). PRRX1 overexpression was found to have the relationship with β-catenin nuclear expression but showed no relationship with Wnt5a and β-catenin cytoplasm expression. Both PRRX1 expression and VM formation were correlated with ovarian cancer metastasis and prognosis. Kaplan-Meier survival analysis showed that patients with PRRX1 expression and VM formation had shorter survival than PRRX1^-or VM^- patients, respectively. And poor prognosiswas observed; PRRX1 expression and β-catenin nuclear expression had shorter survival than PRRX1^-or β-catenin nuclear negative expression (P <0.05),where this relationship showed no significance between PRRX1 and Wnt5a,β-catenincytoplasm expression (P >0.05).Conclusion: PRRX1 could regulate VM formation in ovarian cancer through the activation of Wnt/β-catenin signaling, indicating poor prognosis, so PRRX1 might be a candidate marker for the diagnosis and prognosis predictor of ovarian cancer.