| 胃癌与多肿瘤抑制基因甲基化的研究 |
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| 引用本文： | 全欣鑫,于皆平,沈志祥.胃癌与多肿瘤抑制基因甲基化的研究[J].胃肠病学,1999,4(2):103-104. |
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| 作者姓名： | 全欣鑫 于皆平 沈志祥 |
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| 作者单位： | 湖北医科大学附属第一医院消化内科!430060 |
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| 摘 要： | 目的：探讨抑癌基因ＭＴＳ１ ５’端ＣｐＧ岛异常甲基化与原发性胃癌发病机制之间的关系。方法ＰＣＲ－甲基化检测法研究３１例胃癌标本和１９例正常胃组织中ＭＴＳ１基因５’端ＣｐＧ岛异常甲基化情况。结果：有３５．５％（１１／３１）的胃癌标本和５．３％（１／１９）正常胃组织出现ＭＴＳ１基因５’端ＣｐＧ岛异常甲基化，两者之间有显著性差异（Ｐ＜０．０５）。结论：ＭＴＳ１基因５’端ＣｐＧ岛异常甲基化是其在原发性胃癌中的主要灭活机制，与胃癌的发生发展密切相关。
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| 关 键 词： | 原发性胃癌 异常甲基化 CpG岛 多肿瘤抑制基因 正常 胃组织 MTS1基因 标本 PCR 灭活 |
Inactivation of the Multiple Tumor Suppressor Gene 1 by de novo Methylation in Human Primary Gastric Cancer |
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| Quan Xin-xin,Yu Jie-Ping,Shen Zhi-xiang..Inactivation of the Multiple Tumor Suppressor Gene 1 by de novo Methylation in Human Primary Gastric Cancer[J].Chinese Journal of Gastroenterology,1999,4(2):103-104. |
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| Authors: | Quan Xin-xin Yu Jie-Ping Shen Zhi-xiang |
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| Abstract: | Background/Aims: To study the 5' CPG islands methylation status of the MTS1 gene in human primary gastric cancer. Methods: A PCR assay relying on the inability of some restriction enzymes to cut methylated sequences was used to analyze the methylation status of the MTS1 gene. Results: De novo methylation of the MTS1 gene was observed in 11 of 31(35.5%) primary gastric cancer tissues and in 1 of 19(5.3%) normal gastric mucosa, and there was a significant difference (P<0.05) between them. Conclusions: The results suggest that de novo methylation is predominant inactivation mechanisms of the MTS1 gene and may be associated in the pathogenesis of human primary gastric cancer. |
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| Keywords: | Gastric carcinoma MTS1 gene de novo methylation Inactivation |
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