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| 用HPLC-TOF-MS法分析荷丹片的化学成分 | |
| 引用本文： | 陈昊,刘畅,李燕,吴思,柴逸峰. 用HPLC-TOF-MS法分析荷丹片的化学成分[J]. 药学服务与研究, 2011, 11(6): 439-443 |
| 作者姓名： | 陈昊 刘畅 李燕 吴思 柴逸峰 |
| 作者单位： | 1. 第二军医大学药学院药物分析学教研室,上海200433;第二军医大学,上海200433 2. 第二军医大学药学院药物分析学教研室,上海,200433 3. 北京总后勤部郑常庄干休所卫生所,北京,100141 |
| 基金项目： | 国家自然科学基金资助项目(No.30873196) |
| 摘 要： | 目的:用高效液相色谱-飞行时间质谱(HPLC-TOF-MS)联用技术对荷丹片中化学成分进行鉴别与结构解析。方法:色谱分离采用Agilent Zorbax SB-Aq柱(250mm×4.6mm,5μm),流动相为乙腈(A相)-1%乙酸水溶液(B相),梯度洗脱:0～50min,0%～30%B相,50～90min,30%～75%B相;流速为1ml/min。质谱定性分析采用TOF-MS,在正离子模式下进行扫描,参比离子m/z分别为121.05和922.01。结果:在液质联用条件下,通过TOF-MS以及碎片离子裂解分析,鉴定了荷丹片中的33种化学成分和5组同分异构体。结论:HPLC-TOF-MS联用技术是鉴定荷丹片中化学成分的一种简单、有效的方法。 |
| 关 键 词： | 荷丹片 色谱法,高效液相 质谱法,联用 化学成分 |
Analysis of the chemical constituents of Hedan tablet by HPLC-TOF-MS method |
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| CHEN Hao , LIU Chang , LI Yan , WU Si , CHAI YiFeng. Analysis of the chemical constituents of Hedan tablet by HPLC-TOF-MS method[J]. Pharmaceutical Care and Research, 2011, 11(6): 439-443 | |
| Authors: | CHEN Hao LIU Chang LI Yan WU Si CHAI YiFeng |
| Affiliation: | CHEN Hao1,2,LIU Chang1,LI Yan3,WU Si1,CHAI YiFeng1*(1.Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University,Shanghai 200433,China,2.Undergraduate of Grade 2007,3.Clinic of Zhengchangzhuang Sanatorium for Retired Cadres,Logistics Department of PLA,Beijing 100141,China) |
| Abstract: | Objective：To analyze the chemical constituents of Hedan tablet by high performance liquid chromatographytime of flight-mass spectrometry（HPLC-TOF-MS）. Methods：The separation was performed on a Agilent Zorbax SB-Aq column （250 min× 4. 6 mm, 5μm）. The mobile phase consisting of aeetonitrile（A） -1% acetic acid water solution（B） was used as gradient elute,0-50 min,0% -30%B,50-90 min,30%-75%B. The flow rate was 1 ml/min. TOF-MS was applied for qualitative analysis in positive ion mode with rn/z of reference fragment 121.05 and 922.01. Results： The 33 chemical constituents of Hedan tablet were identified, and 5 groups of isomers were discriminated by TOF-MS and structure-relevant fragment ions. Conclusion： The HPLC-TOF-MS method is simple and reliable for analysis of the chemical constituents of Hedan tablets. |
| Keywords: | Hedan tablet chromatography high performance liquid mass spectrometry tandem chemical constituents |
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