| HPLC同时测定半枝莲中对香豆酸和肉桂酸的含量 |
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| 引用本文： | 付小凯,刘勇,李阳,闫辉,容蓉,巩丽丽,蒋海强,吕青涛.HPLC同时测定半枝莲中对香豆酸和肉桂酸的含量[J].中国实验方剂学杂志,2016,22(3):44-47. |
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| 作者姓名： | 付小凯 刘勇 李阳 闫辉 容蓉 巩丽丽 蒋海强 吕青涛 |
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| 作者单位： | 山东中医药大学药学院, 济南 250355,山东中医药大学药学院, 济南 250355,山东中医药大学药学院, 济南 250355,山东中医药大学药学院, 济南 250355,山东中医药大学药学院, 济南 250355,山东中医药大学药学院, 济南 250355,山东中医药大学药学院, 济南 250355,山东中医药大学药学院, 济南 250355 |
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| 基金项目： | 国家自然科学基金项目(81173614) |
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| 摘 要： | 目的:建立半枝莲药材中同时测定对香豆酸和肉桂酸含量的测定方法。方法:采用Eclipse SB-C18色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈-0.2%磷酸水梯度洗脱,流速1 mL·min-1,检测波长310,280 nm,柱温26℃。结果:对香豆酸和肉桂酸分别在0.148~1.776 μg(r=0.9998),0.062~0.992 μg(r=0.9996)呈良好的线性关系,平均加样回收率分别为99.4%(RSD 1.5%),99.3%(RSD 1.8%),供试品溶液在12 h内稳定。通过对8批半枝莲药材的测定,结果发现湖北(批号130501)样品中2种成分含量总体优于其他产地样品。结论:该含量测定方法精密度良好,准确度高,适用于半枝莲药材中对香豆酸和肉桂酸的含量测定。
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| 关 键 词： | 半枝莲 对香豆酸 肉桂酸 |
| 收稿时间： | 5/5/2015 12:00:00 AM |
Simultaneous Determination of p-coumaric Acid and Cinnamic Acid in Scutellaria barbata by HPLC |
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| FU Xiao-kai,LIU Yong,LI Yang,YAN Hui,RONG Rong,GONG Li-li,JIANG Hai-qiang and LYU Qing-tao.Simultaneous Determination of p-coumaric Acid and Cinnamic Acid in Scutellaria barbata by HPLC[J].China Journal of Experimental Traditional Medical Formulae,2016,22(3):44-47. |
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| Authors: | FU Xiao-kai LIU Yong LI Yang YAN Hui RONG Rong GONG Li-li JIANG Hai-qiang and LYU Qing-tao |
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| Institution: | School of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China,School of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China,School of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China,School of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China,School of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China,School of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China,School of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China and School of Pharmacy, Shandong University of Traditional Chinese Medicine, Ji'nan 250355, China |
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| Abstract: | |
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| Keywords: | Scutellaria barbata p-coumaric acid cinnamic acid |
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