干、鲜壁虎冻干粉对小鼠H22肝癌体内外抑制作用

目的:研究干、鲜壁虎冻干粉对荷H22实体型移植瘤小鼠体内抑瘤作用及其含药血清体外对H22肝癌细胞的增殖抑制作用.方法:采用昆明小鼠H22移植瘤模型观察干、鲜壁虎冻干粉体内抑瘤活性及小鼠胸腺、脾脏指数变化:MTT法检测不同浓度干、鲜壁虎冻干粉含药血清对H22肝癌细胞体外杀伤作用.结果:干、鲜壁虎低、中、高3个剂量组的抑瘤率分别为25.6%,40.0%,48.5%和20.7%,28.1%,51.1%.各壁虎组与西药5-FU组相比,不同程度提高了荷瘤小鼠的胸腺质量(P<0.01)、胸腺指数(P<0.05或P<0.01)和脾脏质量(P<0.05或P<0.01)、脾脏指数(P<0.05).含药血清在体外能够明显抑制H22细胞的增殖,干、鲜壁虎低、中、高含药血清组的增殖抑制率为17.4%,21.0%,34.5%和16.4%,26.3%,43.2%.结论:干、鲜壁虎冻干粉均具有良好的抗肿瘤作用.

Anti-tumor effects of dry and fresh Gekko Swinhonis Gunther freeze-dried powders on mouse H22 hepatocellular carcinoma in vivo and in vitro

AIM:To investigate the anti-tumor activity of dry and fresh Gekko Swinhonis Gunther freeze- dried powder (DGFP and FGFP) on mouse H22 hepatocellular carcinoma in vivo and in vitro. METHODS:The mice bearing H22 tumor cells were used in this study.After treatment with DGFP and FGFP in vivo and in vitro,the anti- tumor effects as well as the weights of thymus and spleen were measured,and the proliferation of H22 cells was detected by MTT assay. RESULTS:The tumor inhibition rates of low-, moderate-and high-dose DGFP were 25.6%, 40.0%,48.5%,while those of FGFP were 20.7%, 27.4%,and respectively,in vivo.As compared with those in 5-FU group,the weights together with the indexes of thymus and spleen were markedly increased (P<0.01 or P<0.05) after DGFP or FGFP treatment.The serum containing DGFP and FGFP obviously restrained the pro- liferafion of H22 cells in vitro,and the inhibition rates of low-,moderate- and high-dose DGFP were 17.4%,21.0%,and 34.5%,while those of FGFP were 16.4%,26.3%,and 43.2%,respectively. CONCLUSION:DGFP and FGFP have conspicu- ous anti-tumor effects.