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| 离体大鼠心脏胰岛素抵抗与心肌损伤 | |
| 引用本文： | 王珏,姚尚龙,伍静,王华荣. 离体大鼠心脏胰岛素抵抗与心肌损伤[J]. 临床麻醉学杂志, 2010, 26(2) |
| 作者姓名： | 王珏 姚尚龙 伍静 王华荣 |
| 作者单位： | 华中科技大学同济医学院附属协和医院麻醉科,武汉市,430022 |
| 基金项目： | 湖北省科技攻关项目(2006AA301B55) |
| 摘 要： | 目的探讨高胰岛素刺激后离体大鼠心脏发生心肌胰岛素抵抗与心肌损伤。方法30只SD大鼠按灌注液中胰岛素含量随机均分为0U/L组(Ⅰ组)、10U/L组(Ⅱ组)、20U/L组(Ⅲ组)、30U/L组(Ⅳ组)和50U/L组(Ⅴ组)五组。采用Langendorff模型以含不同浓度胰岛素的K-H液进行离体心脏灌注。定时搜集并测定冠状动脉流出液样本中葡萄糖浓度,评估心肌葡萄糖氧化情况;测定流出液中肌酸激酶(CK),肌钙蛋白I(cTnI)含量;Western Blot检测心肌细胞胰岛素受体(InsR)含量。结果与Ⅰ组比较,Ⅱ～Ⅴ组大鼠心肌葡萄糖总氧化量明显减少(P<0.05),心肌细胞InsR表达降低,心肌细胞释放CK和cTnI增加(P<0.05)。结论高胰岛素刺激大鼠离体心脏后,心肌细胞出现胰岛素抵抗现象。其程度随胰岛素含量升高而增强,心肌亦受到损伤。 |
| 关 键 词： | 胰岛素抵抗 心肌损伤 |
Myocardial insulin resistance and myocardial damage of isolated rat heart |
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| WANG Jue,YAO Shang-long,WU Jing,et al.. Myocardial insulin resistance and myocardial damage of isolated rat heart[J]. The Journal of Clinical Anesthesiology, 2010, 26(2) | |
| Authors: | WANG Jue YAO Shang-long WU Jing et al. |
| Affiliation: | WANG Jue,YAO Shang-long,WU Jing,et al.Department of Anesthesiology,Union Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430022,China |
| Abstract: | Objective To confirm a hypothesis of myocardial insulin resistance phenomenon on isolated rat heart after stimulated with hyperinsulinism.Methods Thirty SD rats were randomized into five groups according to the concentration of insulin in the buffer. The rats were killed and their hearts were taken out,which were mounted on to a Langendorff perfusion apparatus and perfused with Krebs-Henseleit buffer in the presence of different concentrations of insulin(0,10,20,30,50 U/L). The coronary effluent was collect... |
| Keywords: | Insulin resistance Myocardial damage |
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