环氧化酶-2和表皮生长因子受体在星形细胞瘤中表达的意义

目的探讨环氧化酶-2（COX-2）、表皮生长因子受体（EGFR）在星形细胞瘤中的表达及其相关性。方法用免疫组织化学s—p法检测68例不同病理级别星形细胞瘤组织与5例正常脑组织中COX-2、EGFR及PCNA蛋白的表达，并计算增殖指数（PI），分析COX-2表达与EGFR及PI之间的相关性。结果正常脑组织中COX-2、EGFR表达均为阴性，高度恶性组的COX-2阳性表达率明显高于低度恶性组（73．53％vs 44．18％，P〈0．01），高度恶性组PI（％）明显高于低度恶性组和对照组（46．11±10．68VS23．0g4-6．25，4．52±0．95，P〈0．01）。在低度恶性组与高度恶性组中，COX-2表达阳性的肿瘤其PI明显高于阴性者（P〈0．01）。高度恶性组的EGFR阳性表达率明显高于低度恶性组（67．65％vs38．24％，P〈0．01）。COX-2蛋白在EGFR阳性组的表达明显高于阴性组。结论COX-2、EGFR的表达与星形细胞瘤的病理分级有关，COX-2可促进肿瘤细胞增殖，并与EGFR的表达密切相关，EGFR信号通路可能参与COX-2表达的调控。

Expression of cyclooxygenase-2 and epidermal growth factor receptor in astrocytomas and its clinical significance

Objective To investigate the expression of cyclooxygenase-2 (COX-2) and epidermal growth factor receptor (EGFR) in astrecytomas, as well as the correlation between them. Methods The expression of COX-2, EGFR and PCNA were respectively detected by immunohistochmical (S-P) method in 68 astrocytomas and 5 cases normal brain tissue. Proliferation index (PI) was calculated and the correlation of COX-2, EGFR and PI was analyzed. Results COX-2 and EGFR were negative expression in normal brain tissue. The positive expression rate of COX-2 and EGFR in high grade astrocytomas was significantly higher than that in low grade astrocytomas(73.53% vs 44. 18% ,67.65% vs 38.24%, P <0. 01 ), and the PI was significantly higher than that in low grade astrocytumas as well as normal brain tissue(46.11 ± 10. 68vs 23. 04±6. 25,4. 52±0. 95, P <0. 01 ). The PI in COX-2 positive group was higher than that in negative group( P <0. 01 ). The positive expression rate of COX-2 in the group with EGFR positive expression was higher than that in the negative group. Conclusions The expression of COX-2 and EGFR was related to pathological feature of astrocytomas. COX-2 may promote the proliferation of tumor cells. There was a static correlation between the expression of EGFR and COX-2 in astrocytomas. EGFR signal transduction probably modulated the expression of COX-2 in astrocytomas cells.