PRP促进光老化人皮肤成纤维细胞胶原与透明质酸合成的研究

目的体外研究人皮肤成纤维细胞（HSFs）发生光老化时,富血小板血浆（PRP）对其合成胶原与透明质酸的影响。方法使用紫外线（UVA）照射体外培养的原代人皮肤成纤维细胞,并检测光老化细胞的细胞增殖情况及细胞倍增时间。用含不同浓度PRP（10%、30%、50%）的培养液培养光老化细胞,正常培养的光老化细胞为阴性对照,原代人皮肤成纤维细胞为阳性对照。ELISA检测各组细胞外液中胶原蛋白与透明质酸含量。结果经紫外线照射后,细胞增殖减弱,加入PRP后,伴随PRP浓度的增加,细胞倍增时间缩短;ELISA检测发现,经过UVA照射的成纤维细胞COL1、COL3及透明质酸合成量,显著低于正常培养的成纤维细胞（P〈0.01）。在PRP作用后,成纤维细胞的COL1、COL3及透明质酸合成量均明显增加（P〈0.01）。结论PRP能显著促进光老化人皮肤成纤维细胞胶原与透明质酸合成,可应用于皮肤抗衰老的研究。

The Effect of PRP Promoting the Expression of Collagen and Hyaluronic Acid of Light Aging Human Skin Fibroblasts in Vitro

Objective To explore the effects of platelet-rich plasma(PRP) on collagen and hyaluronic acid(HA) expression of light aging human skin fibroblasts(HSFs) in vitro.Methods Cultured human skin fibroblasts were radiated with ultraviolet ray and the proliferation and doubling time of cell were estimated.Different concentrations(10%,30% and 50%) of PRP were added into cultured light aging human skin fibroblasts,and HSFs radiated with ultraviolet ray were taken as negative control and HSFs were taken as positive control.ELISA was used to measure collagen expression and hyaluronic acid expression.Results The cell proliferation was prohibited after ultraviolet irradiation and cell doubling time was shortened with the increasing of PRP concentration after PRP added.ELISA assay showed that the collagen type 1(COL1),type 3(COL3) and HA expression were decreased after ultraviolet irradiation and promoted after PRP added,significant difference was shown between expermental groups and negative control group(P<0.01).Conclusion PRP can promote the synthesis of collagen type 1(COL1),type 3(COL3) and HA,and may be used as an effective factor against skin photoaging.