前列腺素E1对梗阻性黄疸大鼠肠黏膜损伤的保护作用

目的研究前列腺素E1对梗阻性黄疸大鼠肠黏膜损伤的保护作用。方法将45只Wistar雄性大鼠按随机数表法分为假手术组(Sham组)、梗阻性黄疸组(OJ组)、前列腺E1组(OJ+PGE1组),每组15只,采用胆总管双重结扎建立大鼠梗阻性黄疸模型,术后第7天起,OJ+PGE1组应用前列腺素E1注射液0.1μg·kg^-1·d^-1腹腔注射治疗14 d,其他两组腹腔注射相同剂量生理盐水。3组大鼠分别于术后第21天处死,利用酶联免疫吸附法(ELISA)检测和比较3组大鼠血清内毒素、D-乳酸、TNF-α、IL-6的水平,HE染色观察比较各组大鼠回肠绒毛高度、肠黏膜厚度,免疫组织化学染色和Western-blot检测回肠组织NF-κB蛋白的表达。结果 OJ+PGE1组及OJ组大鼠血浆内毒素、D-乳酸、TNF-α、IL-6的水平表达水平高于Sham组,但OJ+PGE1组增高的程度低于OJ组,差异有统计学意义(P<0.05);与Sham组比较,OJ+PGE1组与OJ组大鼠回肠组织NF-κB蛋白表达明显升高,但OJ+PGE1组增高的程度低于OJ组,差异有统计学意义(P<0.05)。结论前列腺素E1对梗阻性黄疸大鼠肠黏膜损伤具有保护作用,其机制可能与抑制NF-κB的活化和降低炎性因子的表达有关。

The protective effect of prostaglandin E1 on intestinal mucosa in rats with obstructive jaundice

Objective To study the protective effect of prostaglandin E1 on intestinal mucosal lesion in rats with obstructive jaundice. Methods A total of 45 male Wister rats were randomly divided into the sham operation group (SH group), obstructive jaundice group (OJ group) and obstructive jaundice + prostaglandin E1 treatment group (OJ+PGE1 group), with 15 rats in each group. The SH group rats were free without ligation of the common bile duct, OJ group and Group OJ+PGE1 were treated by double thread ligation of the common bile duct, postoperative 1 week, OJ+PGE1 group application of prostaglandin E1 injection (0.1 μg·kg^-1·d^-1) continuous intraperitoneal injection for 2 weeks. The other two groups were intraperitoneally injected with the same dose of normal saline. The three groups of rats were sacrificed on the twenty-first day, expression of serum endotoxin, D-lactic acid, TNF-α, IL-6 were measured by enzyme-linked immunosorbent assay (ELISA), the ileum villus height and thickness of the rats were compared by HE, the expression of nuclear factor-κB (NF-κB) protein were detected by immunohistochemical staining and Western-blot. Results The expression level of serum endotoxin, D-lactic acid, TNF-alpha and IL-6 in the OJ+PGE1 group and OJ group were higher than the Sham group, but the OJ+PGE1 group was significantly lower than the OJ group (P<0.05); compared with the Sham group, the expression of NF-κB protein in the OJ+PGE1 group and OJ group were increased significantly, but the increase in the OJ+PGE1 group was significantly lower than the OJ group (P<0.05). Conclusion Prostaglandin E1 has protective effect on intestinal mucosal injury in rats with obstructive jaundice, and its mechanism may be related to inhibiting the activation of NF-κB and decreasing the expression of inflammatory factors.