肝细胞癌组织Bcl-2和Bax蛋白的表达（英文）

目的　探讨原发性肝细胞癌 (简称肝癌 )细胞凋亡相关蛋白 Bcl- 2和 Bax的表达及其意义 .方法　采用免疫组化ABC法检测 Bcl- 2和 Bax蛋白在 66例人肝癌组织和 2 4例人正常肝组织 ,以及 3株人肝癌细胞系 HCC- 92 0 4 ,SMMC-772 1 ,HHCC和 1株永生化的人正常肝细胞系 QZG的表达情况 ,并分析肝癌组织的 Bcl- 2和 Bax阳性率与其病理分级之间的关系 .结果　 Bcl- 2在正常肝组织的阳性率只有 4.2 % ,而在肝癌组织为 2 5.8%差异显著 (P<0 .0 5) .Bax在正常肝组织的阳性率为 70 .8% ,在肝癌组织为 43.9% ,差异显著 (P<0 .0 5) .肝癌组织的 Bcl- 2和 Bax阳性率与其病理分级之间均未见明显的相关性 (P>0 .0 5) .在正常肝和肝癌组织中 ,Bcl- 2阳性率均明显低于 Bax(P<0 .0 1 ,P<0 .0 5) .在QZG细胞中未见明显的 Bcl- 2阳性信号 ,Bax阳性细胞计数率为 61 % .SMMC- 772 1细胞和 HHCC细胞的 Bcl- 2阳性细胞计数率分别为 1 4 .3%和 1 1 .8% ,Bax阳性细胞计数率分别为 58.3%和 47.1 % .HCC- 92 0 4细胞中未见明显的 Bcl- 2阳性信号 ,Bax阳性细胞计数率为 43.2 % .结论　与正常肝组织相比 ,肝癌组织的 Bcl- 2蛋白低度升高 ,Bax蛋白下降 .正常肝和肝癌组织的 Bcl- 2水平均低于 Bax.肝癌和正常肝细胞系的 Bcl- 2蛋白水平均很

Expressions of Bcl-2 and Bax proteins in hepatocellular carcinoma tissue

AIM To elucidate significance and expression of apoptosis related proteins of Bcl 2 and Bax in primary hepatocellular carcinoma (HCC). METHODS Bcl 2 and Bax proteins were detected in 66 human HCC tissues, 24 human normal liver tissues, 3 human HCC cell lines (HCC 9204, SMMC 7721 and HHCC) and a human immortalized normal hepatocyte cell line QZG by immunohistochemical ABC method. The positive rates and their relationship with pathological grades of HCC tissues were further investigated. RESULTS The positive rate of Bcl 2 was only 4.2% in normal liver tissues, whereas 25.8% (17/66) in HCC ( P <0.05). The positive rate of Bax in normal liver tissues was 70.8%, while that in HCC tissues was 43.9% ( P <0.05). The positive rates of both Bcl 2 and Bax had no distinct correlation with pathological grades of HCC tissues ( P >0.05). The positive rate of Bcl 2 as obviously lower than that of Bax in both normal liver tissues ( P <0.01) and HCC tissues ( P < 0.05). There was no detectable Bcl 2 positive signal, and the counting rate of Bax positive cells was 61% in QZG cells. The counting rates of Bcl 2 positive cells were 14.3% and 11.8%, and those of Bax positive cells were 58.3% and 47.1% in SMMC 7721 cells and HHCC cells, respectively. There was no detectable Bcl 2 positive signal, and the counting rate of Bax positive cells was 43.2% in HCC 9204 cells. CONCLUSION Compared with that in normal liver tissues, the expression of Bcl 2 protein increases a little and Bax protein decreases in HCC tissues. Bcl 2 level is lower than Bax level in both normal liver and HCC tissues. The Bcl 2 protein level is very low and that of Bax is moderate in both HCC and normal hepatocyte cell lines.