| 急性分离的成年大鼠心肌细胞在免疫荧光染色中的应用 |
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| 引用本文： | 陈莹,龚惠,朱依纯.急性分离的成年大鼠心肌细胞在免疫荧光染色中的应用[J].复旦学报(医学版),2005,32(1):110-111,i001. |
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| 作者姓名： | 陈莹 龚惠 朱依纯 |
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| 作者单位： | 复旦大学上海医学院生理与病理生理学系,上海,200032;复旦大学上海医学院生理与病理生理学系,上海,200032;复旦大学上海医学院生理与病理生理学系,上海,200032 |
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| 基金项目： | 国家自然科学基金资助项目(30270548) |
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| 摘 要： | 目的介绍用急性分离的心肌细胞进行免疫荧光染色的方法。方法采用急性分离的成年大鼠心肌细胞和培养的乳鼠心肌细胞为标本，分别用免疫荧光染色直接法和间接法对细胞的肌动蛋白和肌钙蛋白进行染色。结果无论是用肌动蛋白或肌钙蛋白抗体，两种心肌细胞染色效果无明显差别。结论可以尝试用急性分离的成年大鼠心肌细胞为标本进行免疫荧光实验。
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| 关 键 词： | 心肌细胞 细胞培养 免疫荧光 |
| 修稿时间： | 2004年2月19日 |
Immunofluorescence Staining with Acutely Isolated Cardiomyocytes from Adult Rats |
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| CHEN Ying,GONG Hui,ZHU Yi-chun.Immunofluorescence Staining with Acutely Isolated Cardiomyocytes from Adult Rats[J].Fudan University Journal of Medical Sciences,2005,32(1):110-111,i001. |
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| Authors: | CHEN Ying GONG Hui ZHU Yi-chun |
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| Institution: | CHEN Ying,GONG Hui,ZHU Yi-chun Department of Physiology & Pathophysiology,Shanghai Medical College,Fudan University,Shanghai 200032,China |
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| Abstract: | Purpose To describe a method of immunofluorescence staining with acutely isolated cardiomyocytes from adult rats.Methods Acutely isolated myocytes from adult rat and cultured neonatal cardiomyocytes were immunostained with antibodies against to actin and tropon, respectively. Results Both acutely isolated cardiomyocyte and cultured neonatal cardiomyocytes were immunostained with antibodies against actin or troponin. Conclusions Acutely isolated cardiomyocyte can be visualized with immunofluorescence staining. |
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| Keywords: | cardiomyocytes cell culture immunofluorescence |
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