| 侧脑室注射中药提取物三氧化二砷对海马神经元Kv4.2和Kv4.3钾通道表达的影响 |
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| 引用本文： | 王勋,殷昊,周晋,张黎明. 侧脑室注射中药提取物三氧化二砷对海马神经元Kv4.2和Kv4.3钾通道表达的影响[J]. 中医药学报, 2013, 0(5): 66-69 |
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| 作者姓名： | 王勋 殷昊 周晋 张黎明 |
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| 作者单位： | 哈尔滨医科大学附属第一医院,黑龙江哈尔滨150001 |
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| 基金项目： | 黑龙江卫生厅课题项目（12531305）;黑龙江省卫生厅课题项目（2012-520）;黑龙江省博士后启动基金项目（101850）;哈尔滨医科大学第一附属医院院基金项目（2009B17） |
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| 摘 要： | 目的：观察中药提取物三氧化二砷（Arsenic Trioxide,As2O3）对海马神经元Kv4.2、Kv4.3钾通道表达的影响.方法：将SD大鼠随机分为5组,分别经侧脑室注射生理盐水、不同剂量的As2 O3（1mg/mL,0.1mg/mL,0.01 mg/mL）和4-氨基吡啶（4-AP,15mmol/L）,观察给药后大鼠行为学的改变及Westem-blot法检测海马神经元Kv4.2、Kv4.3钾通道的表达.结果：As2 O3随浓度增加,能够剂量依赖性的引起海马神经元Kv4.2、Kv4.3蛋白表达增强,1mg/mL和0.1mg/mL的As2O3可引起大鼠癫痫样的抽搐发作.结论：As2O3能够影响海马神经元Kv4.2、Kv4.3钾通道的表达并引起癫痫样发作的行为学的改变.
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| 关 键 词： | 三氧化二砷 海马神经元 钾通道 侧脑室注射 |
Effect on Expression of Kv4.2 and Kv4.3 of Hippocampus Neurons by Lateral Cerebral Ventricle Injection of Arsenic Trioxide |
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| WANG XunThe First Affiliated Hospital of Harbin Medical University,Harbin,China YIN HaoThe First Affiliated Hospital of Harbin Medical University,Harbin,China ZHOU JinThe First Affiliated Hospital of Harbin Medical University,Harbin,China ZHANG Li-ming. Effect on Expression of Kv4.2 and Kv4.3 of Hippocampus Neurons by Lateral Cerebral Ventricle Injection of Arsenic Trioxide[J]. Acta Chinese Medicine and Pharmacology, 2013, 0(5): 66-69 |
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| Authors: | WANG XunThe First Affiliated Hospital of Harbin Medical University Harbin China YIN HaoThe First Affiliated Hospital of Harbin Medical University Harbin China ZHOU JinThe First Affiliated Hospital of Harbin Medical University Harbin China ZHANG Li-ming |
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| Affiliation: | WANG Xun(The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China) YIN Hao(The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China) ZHOU Jin(The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China) ZHANG Li-ming(The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China) |
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| Abstract: | Objective：To observe the effect of arsenic trioxide （As2O3） on expression of Kv4.2 and Kv4.3 of hippocampus neurons.Methods：The SD rats were divided into five groups randomly,and they were given normal saline,As2 O3 （1 mg/mL,0.1 mg/mL,0.01 mg/mL） and 4-amion pyridine （4-AP,15mmol/L）,respectively.Then the behavioral changes were observed in the rats and expressions of Kv4.2 and Kv4.3 of the hippocampus neurons were observed by western-blot.Results：As2O3 enhanced the expression of Kv4.2 and Kv4.3 of the hippocampus neurons dose dependently with the increase of concentration.As2O3 induced epileptiform seizures of the rats at concentrations of lmg/mL and 0.lmg/mL.Conclusion：As2O3 could affect the expressions of Kv4.2 and Kv4.3 of the hippocampus neurons and induce epileptiform behavioral changes. |
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| Keywords: | Arsenic trioxide Hippocampus neurons Potassium channel Lateral cerebral ventricle injection |
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