紫外线致弱尾蚴免疫兔血清筛选血吸虫成虫cDNA文库

目的 :寻找照射致弱尾蚴中起免疫保护作用的抗原分子 ,为血吸虫病疫苗研究提供新的候选抗原。方法 :用紫外线照射致弱尾蚴免疫兔血清筛选日本血吸虫 (Schistosomajaponicum ,Sj)成虫cDNA文库 ,并对阳性克隆的插入基因片段进行PCR扩增及测序分析。结果 :经三轮筛选 ,获 10个阳性克隆 ,其插入的SjcDNA片段大小为 1 5～1.8kb。初步测序获 5个部分序列 ,其中 2个序列分别与Sj动力蛋白轻链 5 (DLC 5 )及Sj线粒体基因明显同源 ,其余 3个序列为未知的新基因片段。结论 :获得的阳性克隆插入基因片段可能为编码抗日本血吸虫感染的抗原基因

Immunoscreening of Schistosoma japonicum adult worm cDNA library by sera from rabbits vaccinated with ultraviolet-attenuated cercariae

Objective: To find out the major antigenic molecules of radiation attenuated cercariae, and provide some useful candidate antigens for developing schistosomiasis vaccine. Methods: Schistosoma japonicum (Sj) adult worm cDNA library was screened by sera of rabbits vaccinated with ultraviolet attenuated cercariae, and the inserts of positive clones were specifically amplified by PCR and sequenced. Results: Ten positive clones were obtained after three rounds of screening, and the size of Sj cDNA fragments of the positive clones ranged from 1.5?kb to 1.8?kb. Five partial sequences were got after preliminary sequencing. Two of them had significantly homology with Sj dynein light chain 5(DLC 5) gene and Sj mitochondrial gene, respectively, and the others were identified as partial sequences of novel genes for they showed only partial homology with non schistosome genes or other organism in the database. Conclusions: The positive clones may be the genes encoding the antigens that can elicit protective immunity against Sj.