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A LIVER-TUMOR ADHESION PEPTIDE FROM RANDOM PHAGE DISPLAY LIBRARY
引用本文:吴淼,杜冰,汪磊,周忠良,钱旻. A LIVER-TUMOR ADHESION PEPTIDE FROM RANDOM PHAGE DISPLAY LIBRARY[J]. 中国癌症研究, 2006, 18(4): 241-245. DOI: 10.1007/s11670-006-0241-4
作者姓名:吴淼  杜冰  汪磊  周忠良  钱旻
作者单位:School of Life Science, East China Normal University, Shanghai 200062
基金项目:上海科学发展基金;国家自然科学基金;上海市教委资助项目
摘    要:
Objective: To identify and localize the synthesized targeting peptide A54 to liver cancer cell line BEL-7402 in vivo and in vitro for confirming the potential clinical application of peptide A54 in hepatocarcinoma targeting therapy. Methods: Phage A54 was confirmed by ELISA. Biotin and FAM labeled A54 peptides were identified and localized by means of jmmunohistochemistry and immunocytochemistry. Results: A54 peptide could target the liver-rumor tissue in vivo and adhere to several liver-rumor cells in vitro. FAM-labeled A54 peptides were localized on the membrane surface of liver-rumor cells. Conclusion: Synthesized A54 peptide obtained from in vivo phage display technology still kept special abilityto adhere liver-rumor cell in vivo and in vitro. The A54 peptide could be a candidate carrier for hepatocarcinoma targeting therapy.

关 键 词:肝癌 肿瘤 化学治疗 药物治疗 疗效
文章编号:1000-9604(2006)-04-0241-05
收稿时间:2006-07-10
修稿时间:2006-09-28

A liver-tumor adhesion peptide from random phage display library
Miao Wu,Bing Du,Lei Wang,Zhong-liang Zhou,Min Qian. A liver-tumor adhesion peptide from random phage display library[J]. Chinese Journal of Cancer Research, 2006, 18(4): 241-245. DOI: 10.1007/s11670-006-0241-4
Authors:Miao Wu  Bing Du  Lei Wang  Zhong-liang Zhou  Min Qian
Affiliation:(1) School of Life Science, East China Normal University, Shanghai, 200062, China
Abstract:
Objective: To identify and localize the synthesized targeting peptide A54 to liver cancer cell line BEL-7402 in vivo and in vitro for confirming the potential clinical application of peptide A54 in hepatocarcinoma targeting therapy. Methods: Phage A54 was confirmed by ELISA. Biotin and FAM labeled A54 peptides were identified and localized by means of immunohistochemistry and immunocytochemistry. Results: A54 peptide could target the liver-tumor tissue in vivo and adhere to several liver-tumor cells in vitro. FAM-labeled A54 peptides were localized on the membrane surface of liver-tumor cells. Conclusion: Synthesized A54 peptide obtained from in vivo phage display technology still kept special ability to adhere liver-tumor cell in vivo and in vitro. The A54 peptide could be a candidate carrier for hepatocarcinoma targeting therapy.
Keywords:A54 peptide  Identification  localization  Medicine carrier
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