精浆中游离L-肉碱水平与附属性腺生化指标的相关性研究

目的:研究精浆中游离L-肉碱水平与α-葡糖苷酶、果糖、酸性磷酸酶3项附属性腺生化指标之间的相关性,探讨L-肉碱作为一项生化指标在男性生殖功能评价中的作用。方法:采集30例正常生育男性和222例不育患者精液样品,分别以计算机辅助精液分析系统进行精液常规分析,同时测定精浆中游离L-肉碱、果糖水平以及α-葡糖苷酶和酸性磷酸酶活性。用SPSS 12.0统计软件包分析两组间各项生化指标之间的差异,以及精浆中游离L-肉碱与果糖水平、α-葡糖苷酶和酸性磷酸酶活性之间的相关性。结果:正常生育组精浆游离L-肉碱水平明显高于不育组(P<0.01)。两组间α-葡糖苷酶活性差异有统计学意义(P<0.05),而果糖水平和酸性磷酸酶活性差异无统计学意义。相关性分析结果显示,精浆游离L-肉碱水平与精浆中α-葡糖苷酶活性具有较强的正相关关系(r=0.504,P<0.001);而与精浆中果糖水平以及酸性磷酸酶活性之间无相关关系。结论:精浆中游离L-肉碱水平测定可作为评估附睾功能的一项生化指标,其在正常生育组与不育组间的水平差异较两组精浆中α-葡糖苷酶活性差异更为显著,可为男性不育检查及临床诊治和进行有关男性生殖功能机制研究提供参考。

Correlation of Free L-carnitine Level with Accessory Gland Markers and Its Clinical Significance

OBJECTIVE: To evaluate the correlation of the level of free L-carnitine with accessory gland markers and its clinical significance. METHODS: Semen samples from 30 fertile men and 222 infertile patients were collected by masturbation. The measurement of the semen quality was carried out by computer-assisted semen analysis system. The seminal plasma components of free L-carnitine, alpha-glucosidase, fructose and acid phosphatase were determined. The results obtained were statistically calculated with an SPSS 12.0 program to evaluate the difference between the control group and the infertile one and the correlation of the free L-carnitine levels with the seminal plasma components of alpha-glucosidase, fructose and acid phosphatase. RESULTS: The concentration of free L-carnitine (P < 0.01) and the activity of alpha-glucosidase (P < 0.05) were significantly reduced in the infertile group as compared with the control, with no significant difference in the concentration of fructose and the activity of acid phosphatase between the two groups. There was a statistically significant positive correlation between seminal plasma free L-carnitine level and alpha-glucosidase activity (r = 0.504, P < 0.001. CONCLUSION: The determination of free L-carnitine level in seminal plasma is a useful test in the evaluation of epididymal function, which may serve as a guidance for the clinical treatment of male infertility as well as for the study on the mechanisms of male reproduction.