丹参加玻璃酸钠关节内注射对家兔实验性膝骨性关节炎软骨退变的影响

为探讨预防性关节内注射丹参加玻璃酸钠对骨关节炎软骨退变和细胞凋亡的影响,将成年健康日本大耳白兔37只随机分为4组,A组9只,B组9只,C组10只,D组9只。手术切开右后肢膝关节,搜刮髌上囊前壁,推开髌上囊后壁,切除髌下脂肪垫,缝合关节囊及皮肤后关节穿刺,每组每只动物分别注入0.3 ml的生理盐水(A组)、玻璃酸钠注射液(B组)、丹参注射液(C组)、丹参加玻璃酸钠混合液(各0.15 ml,D组),管形石膏固定患肢于屈膝70~90°位;4周后处死动物,行关节腔大体观察,股骨髁下面关节软骨组织学观察和细胞凋亡检测。结果显示,关节软骨退变计分B、C、D组均小于A组((P<0.01),D组的均值小于B、C组(P>0.05);关节软骨凋亡细胞计数B、C、D组均小于A组(P<0.01),D组的均值小于B、C组(P>0.05)。表明丹参能减轻软骨退变程度、减少软骨凋亡细胞数目,有效预防骨关节炎;丹参加玻璃酸钠与二药单独使用比较,有减轻软骨退变程度、减少软骨凋亡细胞数目的趋势,可以提高预防骨关节炎。

PROPHYLACTIC EFFECTS OF INTRA-ARTICULAR INJECTION OF RADIX SALVIAE MILTIONRRHIZAE AND SODIUM HYALURONATE ON CARTILAGE DEGENERATION OF OSTEOARTHRITIS IN RABBIT KNEE JOINTS

In order to investigate the prophylactic effects of intra-articular injection of Radix Salviae MiltioiThizae(RSM) and Sodium Hyaluronate (SH) on cartilage degeneration and cartilage cell apoptosis of osteoarthritis(OA), 37 healthy adult Japanese rabbits were divided randomly into 4 groups: 9 for Group A injected physiological saline 0.3 ml, 9 for Group B injected SH 0.3ml, 10 for Group C injected RSM 0.3 ml and 9 for Group D injected RSM 0.15 ml together with SH 0.15 ml. The right knee joints were made OA model through the way like this: the anterior wall of suprapatellar bursae was scraped while the posterior's was stripped from the femur, and the infrapatellar fat pad behind patellar ligaments was cut off, then the arthicular supsule was sutured and punctured. The knee was immobilized in the flexed position between 70-90 degree with a long leg plaster cast for 4 weeks, then the rabbits were killed, and the inferior articular cartilage of the femoral condoyle was gained for histological analysis and examination of cell apoptosis. The score of articular cartilage degeneration in group B, C or D is smaller than that in group A (P< 0.01), and the mean of scores in group D is smaller than that in group B or C(P>0.05). The number of apoptosis cells of cartilage in group B, C or D is smaller than that in group A (P < 0.05), and the mean of scores of numbers in group D is smaller than that in group B or C (P > 0.05). Similar to SH, RSM can effectively prevent OA; furthermore RSM may strengthen the prophylactic effects of SH on OA.