穿心草山酮对心肌细胞缺氧—再给氧损伤的保护作用

以Ｌａａｒｓｅ‘ｓ方法建立心肌细胞缺氧－再给氧模型，缺糖缺氧６０ｍｉｎ，再给氧３０ｍｉｎ，结果发现心释放ＬＤＨ含量显著增加，细胞膜流动性下降，再给氧组上述各指标改变加剧。山酮能明显提高心肌细胞的成活率，减少乳酸脱氢酶的释放，增加细胞膜流动性，减少心肌细胞膜的损伤。证明有明显抗心肌细胞缺氧－再给氧损伤作用。

Effect of xanthone from Canscora lucidissima on cultured myocytes anoxia-reoxygenation injuries]

The anoxia/reoxygenation model of cultured neonatal rats myocardial cells were developed, according to Laarse's method: anoxia and glucose deficiency for 60 min followed by reoxygenation and re-exposure to glucose for 30 min. The results showed that the survival rate of myocardial cells in the anoxia group was significantly decreased, release of lactate dehydrogenase (LDH) from myocytes was increased and membrane fluidity was decreased, all the changes were much more severe in the reoxygenation group. 30 min before anoxia, addition of xanthones: 1,8-dihydroxy-3, 5-dimethoxyxanthone, 1-hydroxy-3,5-dimethoxyanthone and 1-hydroxy-3,7,8-trithoxyxanthone, isolated from Canscora lucidissima increased the rate of myocardial cells and membrane fluidity, decreased the release of LDH. These results suggested that xanthone may provide some protective effects on the anoxia/reoxygenation damages on myocardium.