人参皂苷Rh2对胶质瘤细胞的体外抑制作用

目的:比较人参皂苷Rh₂、榄香烯与阿霉素对胶质瘤细胞的体外抑制及凋亡诱导作用，以及药物杀伤的时效关系。方法:体外培养胶质瘤细胞, 用MTT肿瘤体外药敏法比较人参皂苷Rh₂、榄香烯与阿霉素对胶质瘤细胞系C6、SHg-44、U-251的抑制作用，并绘制细胞存活率-时间曲线。用流式细胞术检测3种药物作用后细胞凋亡情况。结果：3种药物对胶质瘤细胞系C6、SHg-44、U-251的抑制作用呈明显的剂量依赖性，随浓度的增加而增强。人参皂苷Rh₂对胶质瘤细胞系的抑制作用明显高于榄香烯和阿霉素，与后两者比较差异有显著性(P<0.01)。3种药物对胶质瘤细胞的抑制作用均呈时间依赖关系，随时间延长而增强，且低浓度的人参皂苷Rh₂短时间（12 h）就有生长抑制作用，而榄香烯和阿霉素的起效时间较缓慢。3种肿瘤细胞经低浓度的3种药物作用后均可出现凋亡，其中人参皂苷Rh₂凋亡细胞比率明显高于其他两组，差异有显著性(P<0.01)。结论:人参皂苷Rh₂对胶质瘤具有较好的生长抑制和凋亡诱导作用，起效时间早于榄香烯和阿霉素。人参皂苷Rh₂良好的抑瘤效果和迅速的起效时间具有应用于胶质瘤治疗的潜在价值。 神经胶质瘤,人参皂苷,榄香烯,阿霉素,细胞凋亡

Inhibitory effect of ginsenoside-Rh2 on glioma cells cultivated in vitro

Objective To compare the inhibitory effects of ginsenoside-Rh_2,elemene and adriamycin(AMD) on glioma cells cultivated in vitro.Methods MTT was used to detect the inhibitory effects of ginsenoside-Rh_2,elemene and AMD on glioma cell lines(C6,SHg-44,U251).The survival rate-time curves were established.The abilities of three drugs to induce apoptosis of three cells lines were determined by flow cytometry.(Results The) inhibitory effects of the three drugs on the growth of C6,SHg-44 and U-251 were dose-dependent and could be enhanced with dose increasing.The inhibitory effects of Rh_2 was stronger than that of AMD and elemene on three glioma cell lines.The inhibitory concentration of Rh_2 on three cell lines was significantly lower than that of AMD and elemene(P<0.01),the inhibitory effects of three drugs demonstrated time-and dose-dependency and could be enhanced with time prolonging.Rh_2 exhibited growth inhibition on glioma cells at low concentration in short time(12 h),elemene and AMD took effect slowly,whlie Rh_2 exhibited higher apoptotic rate than other two drugs(P<0.01).Conclusion Ginsenoside-Rh_2 can exhibite excellent activity of growth inhibition and apoptosis induction on glioma cells.It′s recommend for further clinical study in glioma treatment.