骨髓间充质干细胞在纳米晶胶原基骨内生长以及成骨潜能*

摘要 背景：纳米晶胶原基骨修复材料是根据仿生原理制备的纳米骨框架材料，其微结构和成分两方面都与天然骨有相似性 ，具有良好的生物相容性。 目的：研究兔骨髓间充质干细胞与纳米晶胶原基骨修复材料体外复合培养的结合程度，以及构建组织工程骨的可行性。 方法：分离兔骨髓间充质干细胞，体外培养、纯化，取第3代骨髓间充质干细胞与纳米晶胶原基骨修复材料体外复合培养，第3，7，20天后激光共聚焦和扫描电镜观察二者复合程度。 结果与结论：骨髓间充质干细胞和纳米晶胶原基骨修复材料复合良好，共聚焦显微镜和环境扫描电镜观察均可见细胞生长；纳米晶胶原基骨修复材料能够作为良好的支架，它能使骨髓间充质干细胞在其内稳定生长。提示骨髓间充质干细胞在纳米晶胶原基骨修复材料内能很好的生长，并且具有成骨潜能。 关键词：纳米晶胶原基骨修复材料；骨髓间充质干细胞；支架；细胞培养；组织工程骨 doi:10.3969/j.issn.1673-8225.2011.03.003

Growth and osteogenesis potential of bone marrow-derived mesenchymal stem cells in nano-hydroxyapatite/collagen

Abstract BACKGROUND: Nano-hydroxyapatite/collagen (nHAC) is a kind of nano-bone material prepared according to bionic principle, its micro-structure and compositions are closely similar to natural bone, has good biocompatibility. OBJECTIVE: To investigate the bonding of rabbit marrow-derived mesenchymal stem cells (MSCs) and nHAC cultured in vitro, and to explore the feasibility of constructing tissue-engineering bone. METHODS: Rabbit MSCs were isolated, cultured and purified in vitro, then rabbit MSCs of third generation were seeded onto the nHAC. Their adhesion situation was analyzed by laser confocal scanning microscope and scanning electron microscope at 3, 7, 20 days. RESULTS AND CONCLUSION: Rabbit MSCs were well combined with nHAC, cell growth could be seen under confocal scanning microscope and scanning electron microscope; nHAC can be served as a good scaffold, it can make MSCs stably grow. Rabbit MSCs grow adherently in nHAC, and show osteogenesis potential.