| 大鼠肠道菌群对短小蛇根草苷体外代谢转化研究 |
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| 引用本文： | 房卉,李孟璇,李海波,刘文君,孟兆青,黄文哲,王振中,萧伟.大鼠肠道菌群对短小蛇根草苷体外代谢转化研究[J].中国中药杂志,2016,41(10):1921-1925. |
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| 作者姓名： | 房卉 李孟璇 李海波 刘文君 孟兆青 黄文哲 王振中 萧伟 |
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| 作者单位： | 江苏康缘药业股份有限公司, 江苏 连云港 222001,江苏康缘药业股份有限公司, 江苏 连云港 222001,江苏康缘药业股份有限公司, 江苏 连云港 222001,江苏康缘药业股份有限公司, 江苏 连云港 222001,江苏康缘药业股份有限公司, 江苏 连云港 222001,江苏康缘药业股份有限公司, 江苏 连云港 222001,江苏康缘药业股份有限公司, 江苏 连云港 222001,江苏康缘药业股份有限公司, 江苏 连云港 222001 |
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| 基金项目： | 国家“重大新药创制”科技重大专项(2013ZX09402203) |
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| 摘 要： | 为分析短小蛇根草苷经体外大鼠肠道菌群代谢转化的产物,在离体培养的大鼠肠道菌群中,加入短小蛇根草苷,采用HPLC检测代谢进程,采用UPLC-Q-TOF-MS鉴定肠菌代谢转化产物。结果显示,短小蛇根草苷容易被大鼠离体肠道菌群代谢,随着代谢时间的延长,短小蛇根草苷被代谢转化成多个代谢产物,该实验初步鉴定了3个代谢产物。研究结果表明短小蛇根草苷能够在大鼠离体肠道菌群中发生广泛的代谢。
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| 关 键 词： | 短小蛇根草苷 肠道菌群 代谢产物 液质联用 |
| 收稿时间： | 2015/12/8 0:00:00 |
Effect of rat intestinal flora on in vitro metabolic transformation of pumiloside |
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| FANG Hui,LI Meng-xuan,LI Hai-bo,LIU Wen-jun,MENG Zhao-qing,HUANG Wen-zhe,WANG Zhen-zhong and XIAO Wei.Effect of rat intestinal flora on in vitro metabolic transformation of pumiloside[J].China Journal of Chinese Materia Medica,2016,41(10):1921-1925. |
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| Authors: | FANG Hui LI Meng-xuan LI Hai-bo LIU Wen-jun MENG Zhao-qing HUANG Wen-zhe WANG Zhen-zhong and XIAO Wei |
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| Institution: | Jiangsu Kanion pharmaceutical Co., Ltd., Lianyungang 222001, China,Jiangsu Kanion pharmaceutical Co., Ltd., Lianyungang 222001, China,Jiangsu Kanion pharmaceutical Co., Ltd., Lianyungang 222001, China,Jiangsu Kanion pharmaceutical Co., Ltd., Lianyungang 222001, China,Jiangsu Kanion pharmaceutical Co., Ltd., Lianyungang 222001, China,Jiangsu Kanion pharmaceutical Co., Ltd., Lianyungang 222001, China,Jiangsu Kanion pharmaceutical Co., Ltd., Lianyungang 222001, China and Jiangsu Kanion pharmaceutical Co., Ltd., Lianyungang 222001, China |
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| Abstract: | To study the metabolic transformation of pumiloside by rat intestinal flora in vitro and identify its metabolites. Pumiloside was incubated in the rat intestinal flora in vitro. HPLC was used to monitor the metabolic process, and HPLC-Q-TOF-MS was used to identify the structures of biotransformation products. In vitro, pumiloside was easily metabolized by rat intestinal flora, and with the prolongation of metabolic time, pumiloside was transformed into several metabolites. Three metabolites were initially identified in this experiment. The study indicated that pumiloside could be extensively metabolized in the rat intestinal flora in vitro. |
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| Keywords: | pumiloside intestinal flora metabolites LC-MS |
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