In vitro cytotoxicity of six dentin bonding agents |
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| Authors: | E. Koliniotou-Koubia,P. Dionysopoulos,E. A. Koulaouzidou,A. H. Kortsaris,& Y. Papadogiannis |
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| Affiliation: | Dental School, Department of Operative Dentistry and Theagenion Cancer Institute, Aristotle University of Thessaloniki, 54006 Thessaloniki, Greece. |
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| Abstract: | The cytotoxicity of six dentin bonding agents (Syntac, Solobond, Bond 1, Scotchbond 1, Heliobond and F-2000) was tested against an established cell line, L929. Under aseptic conditions 3, 5 and 10 microL dentin bonding agents were placed in the centre of Petri dishes. Each dish was covered with a 5-mL suspension of fibroblasts at a concentration of 40 000 cells mL(-1). The cultures were incubated at 37 degrees C and cytotoxicity was assessed by a quantitative technique at 24 and 72 h. All the dentin bonding agents were found to be cytotoxic. Scotchbond 1 and F-2000 showed the highest cytotoxicity followed by Solobond and Bond 1. Heliobond and Syntac were the least toxic materials. |
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| Keywords: | cytotoxicity denting bonding agents |
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