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Regulation of fibroblast-induced collagen gel contraction by interleukin-1β
Authors:C. R. Irwin  T. Myrillas  M. Smyth  J. Doogan  C. Rice  S. L. Schor
Affiliation:Department of Restorative Dentistry, Queen's University, Belfast, N. Ireland;Department of Dental Surgery and Periodontology, School of Dentistry, University of Dundee, Scotland
Abstract:Fibroblasts incorporated within collagen gels induce a cell-mediated contraction of the gel to form a three-dimensional, tissue-like structure by a mechanism thought to mimic wound contraction in vivo . In this study a gel contraction model was used to investigate the ability of fibroblasts derived from adult gingiva, adult skin and fetal skin to organise a collagen matrix. In addition the effects of interleukin-1β (IL-1β) on the contraction process was also investigated. Over the concentration range 5-50 U/ml, IL-1β induced a statistically significant inhibition of gel contraction in all fibroblast cell types ( P <0.05), although fetal fibroblasts appeared least responsive and gingival fibroblasts most responsive to the inhibitory effects of this cytokine. Comparison of gel contraction by the different fibroblast strains indicated that fetal and gingival fibroblasts shared similar contraction kinetics. For the adult skin fibroblasts, three of five strains studied showed significantly diminished levels of gel contraction compared to fetal and gingival cells. This apparent difference in fibroblast phenotype may, at least in part, explain the fetal-like wound healing pattern seen in the oral mucosa.
Keywords:Key words: fibroblasts    gel contraction    interleukin-1 β    wound healing
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