骨髓增生异常综合征CD3^＋CD4^＋T细胞共刺激分子表达的研究

本研究检测骨髓增生异常综合征（myelodysplastic syndromes，MDS）CD3^＋CD4^＋T细胞共刺激分子的表达，为进一步探讨MDS发病机制积累信息。以38例初治MDS患者为研究对象，依据FAB分型将他们进一步划分为RA／RARS、RAEB／RAEB-t组；以11例献血员为正常对照，应用流式细胞术检测两组外周血CD3^＋CD4^＋T细胞共刺激分子CD28、CD154、CTLA-4、PD-1、CD25的表达。结果表明：MDS组CD28表达下降，CD154升高，CTLA-4、PD-1、CD25表达明显升高。随着疾病恶性程度的增高，RAEB／RAEB-t组CTLA-4、PD-1的表达以及CTLA-4／CD28比值亦较RA／RARS组升高。结论：MDS患者CD3^＋CD4^＋T细胞共刺激分子表达存在异常改变，其表达异常可能在MDS的发病机制中起一定作用。

Expressions of Costimulatory Molecules on CD3+CD4+T Cells in Myelodysplastic Syndrome

The study was aimed to detect the expressions of costimulatory molecules on CD3 CD4 T cells so as to accumulate informations for investigation of mechanism of myelodysplastic syndrome.11 healthy blood donors as control and 38 patients with MDS de novo were studied. 38 MDS patients were divided into RA/RARS group and RAEB/RAEB-t group acconding to FAB classification.The expressions of CD28,CD154,CTLA-4,PD-1,CD25 on CD3 CD4 T cells in peripheral blood were detected by FCM.The results indicated that as compared with normal controls,the expression of CD28 in MDS patients decreased,and CD154 increased.The percentages of CTLA-4,PD-1 and CD25 in MDS were obviously higher than that in normal controls; the differences of CTLA-4,PD-1 and the ratio of CTLA-4/CD28 between RAEB/RAEB-t and RA/RARS were more significant with progressing of MDS.In conclusion,the expressions of costimulatory molecule in MDS patients were abnormal,which may be involved in the pathogenesis of MDS.