舌鳞癌组织中VEGFR-3和淋巴管密度的表达及其临床病理意义

目的：检测舌鳞癌组织中血管内皮生长因子受体3(VEGFR-3)和淋巴管密度(LVD)的表达水平，探讨其与患者临床病理指标的关系。方法选取2006年1月至2014年5月搜集的舌鳞癌标本60例和正常舌体标本12例，采用免疫组化染色法检测组织中VEGFR-3和LVD的表达水平，运用SPSS19.0软件处理实验数据，统计分析VEGFR-3与肿瘤LVD之间的相关性及临床病理意义。结果 VEGFR-3在舌鳞癌组织中的高表达率为56.67%，对照组为25.00%；LVD在舌鳞癌组织中的高表达率为58.33%，对照组为16.67%；舌鳞癌中VEGFR-3和LVD的表达明显高于正常舌组织，差异均具有统计学意义(P<0.05)。VEGFR-3和LVD的高表达与淋巴结转移(PVEGFR-3=0.043，PLVD=0.019)、临床分期(PVEGFR-3=0.006，PLVD=0.015）及生存状态(PVEGFR-3=0.028，PLVD=0.015)之间均具有相关性；VEGFR-3和LVD在舌鳞癌中具有正相关性(r=0.916，P<0.001)。结论 VEGFR-3和淋巴管密度与肿瘤的侵袭、转移及预后密切相关，可作为指导舌鳞癌诊治及判断预后的重要指标。

Expression of VEGFR-3 and LVD in tongue squamous cell carcinoma and their clinical pathological significance

Objective To investigate vascular endothelial growth factor receptor-3 (VEGFR-3) and lymphatic vessel density (LVD) relationships with clinical pathological indicators through studying their expression in tongue squa-mous cell carcinoma. Methods Sixty cases of tongue squamous cell carcinoma (TSCC) and 12 cases of normal tongue were collected from January 2006 to May 2014, and the expression of VEGFR-3 and LVD in these cases were detected by immunohistochemical staining. SSPS19.0 software was used to process the experimental data and analyze the correla-tion between VEGFR-3 and LVD, and their clinical pathological significance. Results The expression rate of VEG-FR-3 in TSCC and normal tongue were respectively 56.67% and 25.00% (P=0.045). The expression rate of LVD in TSCC and normal tongue were respectively 58.33%and 16.67%(P=0.008). The expression of VEGFR-3 and LVD in TSCC were significantly higher than in normal tongue (P<0.05). The expression of VEGFR-3 and LVD were significant-ly associated with lymph node metastasis (PVEGFR-3=0.043, PLVD=0.019), clinical stage (PVEGFR-3=0.006, PLVD=0.015) and liv-ing conditions (PVEGFR-3=0.028, PLVD=0.015). The expression of VEGFR-3 had a positive correlation with the expression of LVD (r=0.916, P<0.001). Conclusion VEGFR-3 and LVD are closely correlated to invasion, metastasis and progno-sis of TSCC. So they can be the important indicators for guiding the diagnosis and treatment, and judging the prognosis of TSCC.