维拉帕米对兔视网膜色素上皮细胞增殖的抑制作用

目的：观察维拉帕米对兔视网膜色素上皮细胞增殖的影响。寻找有效、安全、方便的药物以防治增增性玻璃体视网膜病变。方法：分别将不同浓度的维拉帕米加入体外的兔ＲＰＥ细胞，用噻唑蓝（ＭＴＴ）比色法在自动酶标测定仪测５４０ｍｍ处的吸光值Ａ「旧称光密度（ＯＤ）」，计算出不同浓度条件下的细胞增殖抑制率及５０％抑制浓度，同时用２％台盼蓝色，测定细胞活性。结果：Ｖｅｒ在所设浓度范围内均明显抑制血清诱导的兔ＲＰＥ细胞的

Inhibition effect of verapamil on surum induced proliferation of rabbit retinal pigment epithelial cells in vitro

Objective:To investigate the effect of verapamil on surum induced proliferation of rabbit pigment epithelial (RPE) cells and to search for simple and effective medicine on PVR. Methods:The rabbit RPE cells (passage 2 to 5) were cultured with various concentrations of verapamil in DMEM. The rates of inhibition were respectively determined by MTT method, and the cell viability was estimated by trypan blue exclusion test. Results:Verapamil at all given concentrations significantly inhibited the serum induced proliferation of RPE cells. At concentrations of 80 mg/L and lower, it had no effect on cell viability. The IC 50 of verapamil was 16.7659 mg/L.Conclusion:Verapamil significantly inhibits RPE cell promising drug on PVR.