羟基磷灰石和二氧化钛纳米粒子溶胶的血液相容性

羟基磷灰石 (HAP)和二氧化钛 (TiO2 )纳米粒子是两种具有潜在应用前景的生物材料。本文通过溶血实验、小鼠出血和凝血时间、凝血酶原时间 (PT)和白陶土部分凝血活酶时间 (PTT)测定等对两种纳米粒子溶胶的血液相容性行了初步评价。结果显示 ,HAP纳米粒子溶胶、溶血实验阴性 ,显著延长小鼠的出血、凝血时间和大鼠白陶土部分凝血活酶时间和凝血酶原时间。TiO2 纳米粒子溶胶对小鼠的出、凝血时间无影响 ,溶血实验阴性。对大鼠白陶土部分凝血活酶时间和凝血酶原时间也无影响。两种纳米粒子溶胶均可使体外家兔红细胞发生凝聚。上述实验不同的结果主要与使用了不同的稳定剂有关 :HAP稳定剂为肝素 ,TiO2 稳定剂为聚氯乙烯 (PVC)。提示 ,在考虑纳米粒子溶胶的生物学应用前景时 ,除了纳米粒子本身的性质 ,筛选具备生物学惰性的稳定剂也是不容忽视的问题

Studies on nano-particle sols of hydroxyaptite and titanium dioxide for haemo-compatibility

A biological evaluation is conducted for two types of nano particle sols,hydroxyaptite(HAP) and titanium dioxide(TiO 2).The results show that HAP sol significantly prolongs the bleeding time and coagulation time of mice as well as the prothrombin time(PT)and partial thromboplastin time(PTT) of rats while TiO 2 sol exhibites no such effects.Neither HAP sol nor TiO 2 sol instigated in vitro hemolysis of rabbit erythrocyte.However,both of the materials caused in vitro aggregation of rabbit erythrocytes.The reason underlying the different results as to the two types of material is their specific stablizer,heparin for HAP sol and PVC for TiO 2 sol.We came to the conclusion that a biologically inert stablizer has no less significance than the nano particle's very own nature in a nano material's application prospect.