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| 具有谷胱甘肽过氧化物酶活性的硒化环糊精的中试放大研究 | |
| 引用本文： | 王程,梁冬雪,吕绍武,龚平生,沈娜,吕莉敏,赵杨,徐亚维,赵刚,牟颖,罗贵民,闫岗林. 具有谷胱甘肽过氧化物酶活性的硒化环糊精的中试放大研究[J]. 药物生物技术, 2011, 0(5): 421-425 |
| 作者姓名： | 王程 梁冬雪 吕绍武 龚平生 沈娜 吕莉敏 赵杨 徐亚维 赵刚 牟颖 罗贵民 闫岗林 |
| 作者单位： | 吉林大学分子酶学工程教育部重点实验室;吉林医药学院生化教研室;吉林农业科技学院生物工程系; |
| 基金项目： | 长春市科技局发展计划项目(2009045); 吉林省发展计划重点项目(20100948); 国家自然科学基金项目资助(30970608) |
| 摘 要： | 将-环糊精的2位羟基进行硒化得到的产物(2-SeCD)作为谷胱甘肽过氧化物酶(GPX)的一种人工模拟酶具有广阔的应用开发前景。为了对其进行进一步的研究及生产,需得到大量的产物。在原有的研究基础上,将中试放大实验的剂量扩大为小试实验的50倍,在7次工艺成熟的中试放大实验中获得了大量的2-SeCD。通过碳氢元素分析、红外光谱分析、核磁共振分析、质谱分析等表征实验验证了放大实验所得产物的结构正确,并测定其活力为5.2 U/mol。在2-SeCD对细胞的生物学效应实验中,发现在2-SeCD浓度较低的条件下可降低细胞死亡率而在浓度较高的条件下却抑制细胞生长。通过形态学观察与DNA ladder检测发现高浓度的2-SeCD可诱导Hela细胞凋亡,并经凋亡因子caspase-3的相对活力检测确定此凋亡是由线粒体caspase激活途径诱发的。 |
| 关 键 词： | 硒化环糊精 谷胱甘肽过氧化物酶模拟物 制备 表征 |
Study on Middle Scale Preparation of Selenium Cyclodextrin with Glutathione Peroxidase Activity |
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| WANG Cheng,LIANG Dong-xue,LV Shao-wu,GONG Ping-sheng,SHEN Na,LV Li-min,ZHAO Yang,XU Ya-wei,ZHAO Gang,MU Ying,LUO Gui-min,YAN Gang-lin. Study on Middle Scale Preparation of Selenium Cyclodextrin with Glutathione Peroxidase Activity[J]. Pharmaceutical Biotechnology, 2011, 0(5): 421-425 | |
| Authors: | WANG Cheng LIANG Dong-xue LV Shao-wu GONG Ping-sheng SHEN Na LV Li-min ZHAO Yang XU Ya-wei ZHAO Gang MU Ying LUO Gui-min YAN Gang-lin |
| Affiliation: | WANG Cheng~(1,2),LIANG Dong-xue~1,LV Shao-wu~1,GONG Ping-sheng~1,SHEN Na~1,LV Li-min~1,ZHAO Yang~1,XU Ya-wei~(1,3),ZHAO Gang~1,MU Ying~1,LUO Gui-min~1,YAN Gang-lin~(1*) (1.Key Laboratory of Molecular Enzymology and Engineering of the Ministry of Education,Jilin University,Changchun 130023,China,2.Department of Biochemistry,Jilin Medical College,jilin 132013,3.Department of Bioengineering,Jilin Agriculture Science and Technology College Jilin 132001,China) |
| Abstract: | The 2-hydroxyl of -cyclodextrins can be selenized,producing an artificial simulation GPX enzyme of selenium which has wide application prospects.In order to carry on the further research,a lot of products were needed.Middle scale preparation of 2-SeCD was done based on the experiments of the original research and the amount was expanded for original 50 times.7 experimental results showed that the technologies were perfect and a large number of products were obtained.The structure of product obtained by midd... |
| Keywords: | 2-SeCD Glutathione peroxidase Preparation Characterization |
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