屋尘螨对哮喘患者白细胞介素-10分泌的影响

目的探讨白细胞介素（ＩＬ）１０与支气管哮喘发病的关系，并对其释放影响因素进行研究。方法对２０例过敏性支气管哮喘患者和２０例正常人外周血单个核细胞（ＰＢＬＣ），分别在有无屋尘螨（ＨＤＭ）和氟美松作用下进行体外培养，采用双抗体夹心酶联免疫吸附实验法对培养上清液中ＩＬ１０进行测定。结果（１）哮喘患者ＰＢＬＣ培养液中ＩＬ１０浓度为（４４．６±２．５）ｎｇ／Ｌ，正常对照为（５３．６±８２）ｎｇ／Ｌ，两组比较差异有显著性（Ｐ＜０．０１）。（２）加入ＨＤＭ后ＩＬ１０分泌进一步受到抑制，而正常对照无明显变化。（３）在哮喘组加入氟美松后ＩＬ１０释放显著增加，正常对照也明显增加。结论ＰＢＬＣ释放ＩＬ１０减少与哮喘发病有关，ＨＤＭ和氟美松对ＩＬ１０的释放有影响。

A study on the effect of house dust mite on inteleukin 10 secretion of peripheral blood lymphocytes in patients with allergic asthma

Objective To investigate the relationship between interleukin (IL)10 and allergic asthma, and the effect of antigen on IL10. Methods The supernatant of cultured peripherial blood lymphocytes (PBLC) from 20 patients with allergic asthma and 20 normal subjects with defferent stimuli (HDM) or dexamethasone] was determined for IL10, by means f enzymelinked immunosorbent assay. Results (1) IL10 was significantly decreased in asthma group, as compared with the control group (P<0.01); (2) Less IL10 was released in HDMstimulated PBLC in asthma (P<0.01); (3) IL10 was obviously increased in dexamethasonstimulated whether in the asthma or in the control group. Conclusion The diminished IL10 production from PBLC could be related to asthmatic attack in patients with allergic asthma. Dexamethason might exert, at least, a part of antiinflammatory activity by inducing IL10 secretion of PBLC.