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Isolation of embryonic chick myosatellite and pluripotent stem cells
Authors:Young  Henry E.  Ceballos  Elizenda M.  Smith  Jennifer C.  Lucas  Paul A.  Morrison  Donna C.
Affiliation:(1) Department of Surgery, Mercer University School of Medicine, 31207 Macon, Georgia;(2) Department of Biology, Wesleyan College, 31297 Macon, Georgia;(3) Department of Surgery, Medical Center of Central Georgia, 31206 Macon, Georgia;(4) Division of Basic Medical Sciences, Mercer University School of Medicine, 1550 College Street, 31207 Macon, GA
Abstract:
Summary The current study outlines the isolation and culture of two populations of cells derived from Day 11 embryonic chick leg muscle and associated connective tissues. The two populations consisted of myogenic lineage-committed stem cells (myosatellite stem cells) and lineage-uncommitted stem cells (pluripotent stem cells). After long-term culture the lineage-uncommitted stem cell population displayed differentiated phenotypes suggestive of the following adult tissues, fibroblasts, muscle, fat, cartilage, and bone.
Keywords:isolation  myosatellite stem cells  pluripotent stem cells  myogenic  uncommitted  chick
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