| 反式二羟环氧苯并芘对人支气管上皮细胞中HER2/neu基因表达的影响 |
| |
| 引用本文： | 傅娟,蒋义国,邹云锋,沈月兰,周兰兰,陈学敏. 反式二羟环氧苯并芘对人支气管上皮细胞中HER2/neu基因表达的影响[J]. 卫生研究, 2007, 36(6): 657-659 |
| |
| 作者姓名： | 傅娟 蒋义国 邹云锋 沈月兰 周兰兰 陈学敏 |
| |
| 作者单位： | 1. 华中科技大学同济医学院劳动卫生与环境卫生学系教育部环境与健康重点实验室,武汉,430030
2. 广州医学院化学致癌研究所 |
| |
| 基金项目： | 国家自然科学基金;教育部留学回国人员科研启动基金 |
| |
| 摘 要： | 目的探讨环境致癌物苯并(a)芘代谢物反式二羟环氧苯并芘(BPDE)对人支气管上皮细胞HER2/neu基因表达的影响。方法利用半定量RT-PCR、SYBR GreenI实时定量RT-PCR(QRT-PCR)、Western blot及免疫细胞化学方法分别检测经2.0μmol/L反式BPDE诱发人支气管上皮细胞恶性转化细胞(16HBE-T)与对照DMSO溶剂组未恶性转化细胞(16HBE-N)之间HER2/neu基因mRNA和蛋白表达水平的差异,及两组细胞内HER2/neu蛋白表达定位分析。结果经几种不同方法检测反式BPDE恶性转化人支气管上皮细胞中HER2/neu基因mRNA和蛋白水平都比对照溶剂组细胞(16HBE-N)表达显著升高(P<0.05)。HER2/neu蛋白定位在胞膜。结论反式BPDE诱发人支气管上皮细胞恶性转化存在HER2/neu表达增高,其可能与原癌基因HER2/neu被激活作用有关。
|
| 关 键 词： | 反式二羟环氧苯并(a)芘 人支气管细胞 HER2/neu基因表达 |
| 文章编号： | 1000-8020（2007）06-0657-03 |
| 收稿时间： | 2007-02-10 |
| 修稿时间： | 2007-02-10 |
Effect of anti-BPDE on expression of HER2/neu gene in the human bronchial epithelial cell line |
| |
| FU Juan, JIANG Yiguo, ZOU Yunfeng, SHEN Yuelan,et al.. Effect of anti-BPDE on expression of HER2/neu gene in the human bronchial epithelial cell line[J]. Journal of hygiene research, 2007, 36(6): 657-659 |
| |
| Authors: | FU Juan JIANG Yiguo ZOU Yunfeng SHEN Yuelan et al. |
| |
| Affiliation: | Department of Occupational and Environmental Health, MOE Key Lab of Environment and Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. fjuangy@126.com |
| |
| Abstract: | OBJECTIVE: To explore the effects of anti-benzo(a)pyrene-trans-7,8-dihydrodiol-9,10-epoxide (anti-BPDE) on expression of HER2/neu gene in the human bronchial epithelial cells. METHODS: The levels of HER2/neu mRNA and protein expressions, and its protein localization were examined in the malignant transformed 16HBE (16HBE-T) induced by 2.0 micromol/L anti-BPDE and untransformed control 16HBE (16HBE-N) using semi-quantitative RT-PCR, SYBR Green I Real time quantitative RT-PCR (QRT-PCR), western blot and immunocytochemical method respectively. RESULTS: The expressions of mRNA and protein of HER2/neu appeared to be a significant increase in 16HBE-T in comparison with those of 16HBE-N detected by several assays. HER2/neu protein showed membrane localization. CONCLUSION :HER2/neu could be overexpressed on treatment of human bronchial epithelial cells with anti-BPDE. The activity of HER2/neu oncogene might play an important role in the malignant transformed 16HBE induced by anti-BPDE. |
| |
| Keywords: | anti-BPDE human bronchial epithelial cell HER2/neu gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
