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| 乳腺癌干细胞微球体形成的影响因素 | |
| 引用本文： | 黄明主,张凤春,张雁云. 乳腺癌干细胞微球体形成的影响因素[J]. 北京大学学报(医学版), 2008, 40(5): 500-504 |
| 作者姓名： | 黄明主 张凤春 张雁云 |
| 作者单位： | (1.上海交通大学医学院附属仁济医院肿瘤科,上海 200127;2.中国科学院上海生命科学研究院/上海交通大学医学院健康科学研究所免疫学联合实验室) |
| 基金项目： | 国家自然科学基金，上海市科委资助项目，上海交通大学医学院肿瘤研究合作中心基金 |
| 摘 要： | 目的:探讨MCF-7、MDA-MB-231及原代乳腺癌细胞微球体形成的影响因素,并检测其中CD44+/CD24-/low的表达.方法:将MCF-7、MDA-MB-231及原代乳腺癌细胞进行微球体培养.取培养第7天的微球体细胞,用流式细胞术分析干细胞比例.结果:MCF-7形成微球体的效率最高(2.1%±0.3%),MDA-MB-231很少能形成微球体,原代乳腺痈细胞中未观察到微球体形成.但在无B27的干细胞培养环境中,MCF-7易贴壁生长.MCF-7单层培养细胞中CD44+/CD24-/low的比例为2.0%±0.1%,将悬浮及贴壁来源的McF-7细胞进行微球体培养,微球体细胞中CD44+/CD24-/low的比例分别为11.8%±0.3%和8.2%±0.8%(P<0.01).MDA-MB-231及原代乳腺癌细胞中CD44+/CD24-/low的表达比例分别为92.2%±3.1%和93.8%±2.4%.结论:MCF-7细胞通过微球体培养富集了肿瘤干细胞,而MDA-MB-231及原代乳腺癌细胞则不能.MDA-MB-231及原代乳腺癌细胞中CD44+/CD24-/low的表达与微球体形成不呈正相关. |
| 关 键 词： | 乳腺肿瘤 肿瘤干细胞 细胞培养技术 |
Influence factors on the formation of mammospheres from breast cancer stem cells |
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| HUANG Ming-zhu,ZHANG Feng-chun,ZHANG Yan-yun. Influence factors on the formation of mammospheres from breast cancer stem cells[J]. Journal of Peking University. Health sciences, 2008, 40(5): 500-504 | |
| Authors: | HUANG Ming-zhu ZHANG Feng-chun ZHANG Yan-yun |
| Affiliation: | HUANG Ming-zhu1,ZHANG Feng-chun1,ZHANG Yan-yun2(1.Department of Oncology,Renji Hospital,School of Medicine,Shanghai Jiaotong University,Shanghai 200127,China,2.Joint Immunology Laboratory,Institute of Health Sciences,Shanghai Institute for Biological Sciences,Chinese Academy of Sciences & School of Medicine,Shanghai Jiaotong University) |
| Abstract: | Objective:To investigate the influential factors on the formation of mammospheres from MCF-7,MDA-MB-231 and primary breast cancer epithelial cells,and to detect the proportion of CD44+/CD24-/low among them.Methods:MCF-7,MDA-MB-231 and primary breast cancer epithelial cells were cultured in suspension to generate primary mammospheres.After 7 days of cultivation,the proportion of cancer stem cells was measured in cells derived from mammosphere cells or monolayer culture cells by flow cytometry.Results:MCF-7 h... |
| Keywords: | Breast neoplasms Tumor stem cells Cell culture techniques |
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