柠檬酸钠联合三氧化二砷体外抗人胃癌细胞及其作用机制

目的探讨柠檬酸钠（Citrate）与三氧化二砷（As2O3）联合应用对胃癌细胞的凋亡及其作用机制。方法用Citrate（终浓度为5 mmol/L）和（或）不同浓度的As2O3（终浓度依次为5、10 μmol/L）处理体外传代培养的胃癌细胞株SGC-7901和BGC-803。流式细胞仪检测细胞凋亡和细胞周期的变化;RT-PCR法观察凋亡相关基因Bcl-2、Mcl-1表达的变化。结果Citrate与As₂O3联合应用相对于单用Citrate或As₂O3可显著提高诱导胃癌细胞凋亡率（P<0.05）;与空白组相比，用药后 G0／G₁期细胞比例下降，G₂/M期细胞比例上升,细胞周期阻滞于G₂/M期，抗凋亡基因Bcl-2、Mcl-1表达明显下降。结论Citrate与As2O3联合应用具有协同抗胃癌细胞的作用,其机制可能与细胞周期阻滞、增强诱导胃癌细胞凋亡以及调控凋亡相关基因的表达有关。

Anti-tumor Effects and Mechanism of Arsenic Trioxide in Combination with Sodium Citrate in Gastric Cancer Cells in vitro

Objective
To study the anti-tumor effects of citrate combined with arsenic trioxide on human gastric cancer cell in vitro and possible mechanism.MethodsCitrate (final concentration of 5 mmol / L) and (or) different concentrations of As₂O3 (final concentration was 5 or 10 μmol / L) treated in vitro cultured gastric cancer cell line SGC-7901 and BGC-803.Flow cytometry was used to detect apoptosis and cell cycle changes.The apoptosis-related gene Bcl-2 and Mcl-1 expressions were identified by RT-PCR to observe.ResultsCitrate combining with As₂O3 had a stronger inhibition function on human gastric cancer cell line compared with citrate or As₂O3(P<0.05) alone.Compared with blank group, the cell cycle distribution was arrested at G₂ / M phase, and the proportion of G0/G1 phase cell decreased and G2/M phase increased (P<0.05).The expression of bcl-2 and mcl-1 was significantly decreased（P<0.05）.ConclusionCitrate combining with As2O3 had the synergistic effects on gastric cancer cell.The mechanism might be related to cell cycle blocking and apoptosis-inducing effects through bcl-2 and mcl-1 genes.