| HPLC法测定抗感解热颗粒中葛根素含量 |
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| 引用本文： | 曲雷鸣,龚伟. HPLC法测定抗感解热颗粒中葛根素含量[J]. 辽宁中医药大学学报, 2014, 0(6): 62-63 |
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| 作者姓名： | 曲雷鸣 龚伟 |
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| 作者单位： | 辽宁中医药大学附属医院,辽宁沈阳110032 |
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| 摘 要： | 目的:利用HPLC法对抗感解热颗粒中葛根素的含量进行测定。方法:采用HPLC法,DiamonsilC18色谱柱(250mm×4.6mm,5μm),流动相为甲醇-水(24∶76),检测波长为250nm,流速为1.0mL/min。结果:葛根素在0.1841~1.841μg范围内呈良好的线性关系(r=0.9998),RSD为1.65%(n=6)。葛根素平均回收率为99.32%。结论:本方法简便、可靠、准确,可用于抗感解热颗粒的质量控制。
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| 关 键 词： | 抗感解热颗粒 葛根素 HPLC 含量 |
Determination of Puerarin in Kanggan Jiere Granule by HPLC |
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| QU Leiming,GONG Wei. Determination of Puerarin in Kanggan Jiere Granule by HPLC[J]. Journal of Liaoning University of Traditional Chinese Medicine, 2014, 0(6): 62-63 |
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| Authors: | QU Leiming GONG Wei |
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| Affiliation: | ( The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China ) |
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| Abstract: | Abstract： Objective： An HPLC-UV method was established to determine puerarin in Kanggan Jiere Granule. Methods ： Chromatographic separation was achieved on a Diamonsil Cla column （250 mm ×4.6 mm, 5 μm）. The mobile phase consisted of methanol and water （ 24 ： 76, v/v ） at a flow-rate of 1.0 mL ·min-1. The ultraviolet detector operated at 250 nm. Results： Linear calibration curves were obtained over the concentration range of 0.1841-1.841 μg · mL for puerarin （ r= 0.9998 ）, RSD=1.65% （ n=6 ）. The average recovery rate was 99.32% with RSD of 1.65%. Conclusion ： The method is simple, reliabe, accurate and it could be used in the determination of puerarin in Kanggan Jiere Granule. |
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| Keywords: | Kanggan J/ere Granule puerarin HPLC content determination |
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