浙南地区HBV基因型分布特征及其与慢性乙型肝炎患者血清HBV DNA水平和HBeAg表达的关系

目的分析浙南地区慢性乙型肝炎患者HBV基因型分布特征,探讨不同基因型与患者血清中HBV DNA水平及HBeAg表达的关系。方法收集2011年6月-2012年3月161例就诊的HBV DNA阳性患者血清标本,采用型特异性引物PCR方法对HBV进行基因分型并用实时荧光定量PCR方法测量HBV DNA含量(拷贝/ml)和ELISA方法检测HBeAg;对HBV DNA含量进行对数转换使其符合正态分布后,运用χ2检验分析基因型与HBeAg阳性率的关系,t检验分析B、C基因型患者HBV DNA水平的差异。结果 161例患者中,B型41例,占25.5%;C型118例,占73.3%;BC混合型2例,占1.2%。C型患者HBV DNA水平和HBeAg阳性率分别为(5.84±1.40)log10拷贝/ml和64.4%,高于B型的(5.49±1.33)log10拷贝/ml和53.7%,但差异均无统计学意义(P0.05)。结论浙南地区HBV基因型以C型为主,B型次之。C基因型HBV DNA水平与HBeAg阳性率均高于B基因型,肝硬化的发生与C基因型HBV密切相关。

HBV genotype distribution and its relationship with serum HBV DNA level and HBeAg expression among patients with chronic hepatitis B in southern Zhejiang Province,China

Objective To analyze the hepatitis B virus （HBV）genotype distribution among chronic hepatitis B patients in southern Zhe-jiang Province,China,and to explore the relationship of HBV genotype with serum HBV DNA level and HBeAg expression.Methods Ser-um samples were collected from 161 patients positive for HBV DNA who visited our hospital from June 2011 to March 2012.Sequence -spe-cific primer PCR was used for the HBV genotyping;real -time quantitative PCR was used to quantify HBV DNA （copies/ml）;ELISA was used to detect HBeAg.After logarithmic transformation was performed on HBV DNA levels so that the data were in normal distribution,the relationship between HBV genotype and HBeAg positive rate was analyzed by chi -square test,and the difference in HBV DNA level be-tween patients with B and C genotypes was analyzed by t test.Results Of the 161 patients,41 （25.5%）had B genotype,118 （73.3%） had C genotype,and 2 （1.2%）had B /C mixed genotype.The HBV DNA level and HBeAg positive rate for patients with C genotype were 5.84 ±1.40 log10 copies/ml and 64.4%,respectively,higher than those for patients with B genotype （5.49 ±1.33 log10 copies/ml and 53.7%）.Conclusion In southern Zhejiang Province,C genotype is the predominant genotype of HBV,followed by B genotype.Both HBV DNA level and HBeAg positive rate are higher in patients with C genotype than in those with B genotype.C genotype is closely associat-ed with the development of liver cirrhosis.