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| 用HPLC法测定芪归生血胶囊中黄芪甲苷的含量 | |
| 引用本文： | 夏爱军,黄贵明. 用HPLC法测定芪归生血胶囊中黄芪甲苷的含量[J]. 药学服务与研究, 2008, 8(4): 264-265 |
| 作者姓名： | 夏爱军 黄贵明 |
| 作者单位： | 1. 中国人民解放军第303医院药剂科,南宁,530021 2. 广西医科大学制药厂销售部,南宁,530021 |
| 摘 要： | 目的：建立芪归生血胶囊中黄芪甲苷（astragaloside Ⅳ）含量测定的HPLC法。方法：采用Hypersil C18柱（250mm×4．6mm，5μm），以乙腈-水（38：62）为流动相，流速为1．0mL／min，检测波长为203nm，柱温为25℃。结果：黄芪甲苷线性范围为0．0403～0．3628mg／mL。平均加样回收率为97．5％，RSD为1．1％（n=5）。精密度、稳定性和重现性实验结果均符合方法学要求。结论：本方法灵敏、准确，重复性好，可用于芪归生血胶囊中黄芪甲苷的含量测定。 |
| 关 键 词： | 芪归生血胶囊 黄芪甲苷 色谱法 高压液相 |
Determination of the content of astragaloside Ⅳ in Qiguishengxue capsule by HPLC method |
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| XIA Ai-jun,HUANG Gui-ming. Determination of the content of astragaloside Ⅳ in Qiguishengxue capsule by HPLC method[J]. Pharmaceutical Care and Research, 2008, 8(4): 264-265 | |
| Authors: | XIA Ai-jun HUANG Gui-ming |
| Affiliation: | XIA Ai-jun1,HUANG Gui-ming2(1.Department of Pharmacy,the 303rd Hospital of PLA,Nanning 530021,China,2.Department of Sales,Pharmaceutical Factory of Guangxi Medical University,China) |
| Abstract: | Objective： To establish a HPLC method for determination of the content of astragaloside Ⅳ in Qiguishengxue capsule. Methods： Hypersil C18 column （250 mm× 4.6 mm,5 μm） was used with acetonitrile-water（38 ： 62） as the mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was 203 nm and the column temperature was 25 ℃. Results： The linear range of astragaloside IV was 0. 040 3 -0. 362 8 mg/mL. The average recovery rate was 97.5% and RSD was 1.1% （n=5）. The results of precision, stability and reproducibility could meet the requirements of methodology. Conclusion： The method is sensitive, accurate and has good reproducibility. It can be applied to the determination of astragaloside IV in Qiguishengxue cap sule. |
| Keywords: | Qiguishengxue capsule astragaloside Ⅳ chromatography, high pressure liquid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |