复方葛根合剂及葛根中葛根素含量的高效液相色谱分析

 目的为了控制复方葛根合剂和葛根药材的质量，需要建立其中有效成分葛根素的含量测定方法。方法采用高效液相色谱法，考察了样品制备方法、流动相系统、检测波长等因素对分析结果的影响，色谱柱为Shim Pack CLC-ODS(6.0 mm×150 mm)不锈钢柱，以甲醇-水(29∶71)为流动相，流速1 mL·min^-1，检测波长306nm，进样量10μL。结果葛根素和其它组分可达基线分离，葛根素浓度在20.0～180.0 μg·mL^-1内线性关系良好(r=0.99998)，合剂和生药的回收率分别为99.60%和100.4%，RSD＜1.6%，合剂经简单的稀释和过滤后即可直接进样测定。结论此法操作简便，结果准确可靠，精密度好。

Study on determination of puerarin in compound Gegen mixture and Gegen crude drugby HPLC

OBJECTIVE To establish a method for the determination of the active constituent puerarin in compound Gegen mixture and Gegen crude drug.METHODS A simplified HPLC procedure was developed.The effects of sample preparation,the mobile phase and detection wavelength on the separation of the various components in samples were investigated.A 6.0 mm×150mm Shim-Pack CLC-ODS column with a mobile phase consisting of methanol-H₂O(29∶71) was used.It was detected at 306 nm.RESULTS Puerarin and other components were well separated.The standard curve was linear over the concentration range of 20.0～180.0μg·mL^-1and the correlation coefficient was 0.99998.The recoveries of puerarin were 99.60% for compound Gegen mixtyre and 100.4% for Gegen crude drug with RSD less than 1.6%.Compound Gegen mixture could be directly chromatographed after it was diluted by CH₃OH-H₂O(50∶50) and filtrated.CONCLUSION The proposed method was simple,accurate and precise.