| 鼠脊神经节硫胺素单磷酸酶阳性神经元的P物质免疫反应及外周投射 |
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| 引用本文： | 王进堂,张祜曾,王健本. 鼠脊神经节硫胺素单磷酸酶阳性神经元的P物质免疫反应及外周投射[J]. 华中科技大学学报(医学版), 1992, 0(1) |
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| 作者姓名： | 王进堂 张祜曾 王健本 |
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| 作者单位： | 同济医科大学基础医学部解剖学教研室(王进堂，张祜曾)，同济医科大学基础医学部解剖学教研室(王健本) |
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| 基金项目： | 国家自然科学基金资助课题 |
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| 摘 要： | 用酶组化、免疫组化和辣根过氧化物酶(HRP)追踪法探讨大鼠脊神经节内硫胺素单磷酸酶(TMPase)和P物质(SP)的定位关系,胃幽门部是否接受TMPase阳性神经支配.在C5～8脊神经节内,一些小型神经元有TMPase活性,SP阳性神经元主要为小型,绝大多数TMPase阳性神经元不具有SP-IR;另有一部分小型神经元既无TMPase活性,又无SP-IR。将CB-HRP注于幽门部,在双侧T_6～L_1脊神经节见少量HRP标记细胞,约4％HRP标记细胞为TMPase阳性。
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| 关 键 词： | 硫胺素单磷酸酶 P物质 辣根过氧化物酶 幽门 脊神经节 |
Peripheral Projections and Substance P-Immunoreactivity of Thiamine Monophosphatase-positive Neurons of Spinal Ganglia in Rats |
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| Wang Jintang,Zhang Huzeng,Wang Jianben. Peripheral Projections and Substance P-Immunoreactivity of Thiamine Monophosphatase-positive Neurons of Spinal Ganglia in Rats[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1992, 0(1) |
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| Authors: | Wang Jintang Zhang Huzeng Wang Jianben |
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| Affiliation: | Wang Jintang,Zhang Huzeng,Wang JianbenDepartment of Anatomy,Faculty of Basic Medical Sciences,Tongii Medical University,Wuhan |
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| Abstract: | The localization of thiamine monophosphatase (TMPase) and sub-Stance P (SP) and the TMPase-positive innervation of pylorus were separately investigated by means of enzymohistochemical method, immunocytochemical method and horseradish peroxidase (HRP) tracing technique. In C5-8 ganglia, a portion of the small-sized neurons displayed TMPase activity and. another portion was SP-immunoreactive (SP-IR). A few of the TMPase-positive neurons were SP-IR, most were not. A portion of the small-sized neurons displayed neither TMPase activity nor SP-IR. In bilateral Te-L1 ganglia, a small number of HRP-labeled cells were observed. A small portion (4%) of the labeled cells displayed TMPase activity. TMPase and SP were mainly located in different subpopulations. of ganglion neurons. However, a majority of spinal ganglion neurous innervating pylorus displayed no TMPase activity. |
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| Keywords: | thiamine monophosphatase substance P horseradish peroxidase pylorus spinal ganglia |
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