| 硝酸纤维素膜上化学药物交联抗原在斑点酶免疫法中的应用 |
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| 引用本文： | 潘培根,俞红吉,曾蓉,杨继虞.硝酸纤维素膜上化学药物交联抗原在斑点酶免疫法中的应用[J].四川大学学报(医学版),1998(2). |
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| 作者姓名： | 潘培根 俞红吉 曾蓉 杨继虞 |
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| 摘 要： | 在硝酸纤维素膜上用４０％酒精直接交联方式和自然渗滤吸附方式固定抗原后，分别作斑点酶免疫法检测血清中的ＨＣＭＶＩｇＧ。结果显示：前者的重复性（ＣＶ＝６７％）优于后者（ＣＶ＝２５５％）。酒精交联抗原斑点酶免疫法与ＥＬＩＳＡ法比较，血清中ＨＣＭＶＩｇＧ检测的一致率为９３２％，阳性血清、弱阳性血清和阴性血清的检测率无显著性差异。以ＥＬＩＳＡ法为金标准，酒精交联抗原班点酶免疫法的敏感性、特异性、诊断指数和诊断效率分别为１００％、９６６％、１９６６％和９８７％，这些质量指标均达到实际应用标准。酒精交联抗原操作简便，快速，重复性好，节省材料，质量指标高，适合临床检验室应用
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| 关 键 词： | 化学药物 抗原交联 斑点酶免疫法 |
Application of Antigen Linked with Chemicals on Nitrate Cellulose Membrane for Dot Enzyme Immunoassay |
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| Pan Peigen,Yu Hongjie,Zeng Rong,Yang Jiyu.Application of Antigen Linked with Chemicals on Nitrate Cellulose Membrane for Dot Enzyme Immunoassay[J].Journal of West China University of Medical Sciences,1998(2). |
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| Authors: | Pan Peigen Yu Hongjie Zeng Rong Yang Jiyu |
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| Institution: | Chengdu 610041 |
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| Abstract: | HCMV IgG in sera of pregnant women was detected by means of dot enzyme immunoassay(DEIA), in which antigen was cross linked on nitrate cellulose membrane through chemical reagent.The results showed better repetition by DEIA,compared with ELISA,and no significant differences were noted between the two methods in the rates of detecting HCMV IgG in positive,weak positive and negative sera.The rate of agreement was 93.2%.In examining the sera which had been examined by ELISA for three times with the same,results the sensitivity,specificity,index and efficiency of diagnosis by DEIA were 100%,96 6%,196 6% and 98.7% respecitively.Since this method is convenient,fast,of good repetition,economical and its diagnosis index meets the application criterion,it is more suitable for clinical laboratories. |
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| Keywords: | Chemicals Antigen cross linking Dot enzyme immunoassay |
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