山楂叶化学成分的分离与鉴定

目的对蔷薇科山楂属植物山楂(Crataegus pinnatifida Bge.)叶的化学成分进行研究。方法运用硅胶柱色谱﹑Sephadex LH-20柱色谱﹑ODS柱色谱、制备HPLC等分离手段进行化学成分的分离纯化,根据理化性质及波谱数据鉴定其结构。结果从山楂叶体积分数80%乙醇水溶液的提取物中分离得到7个化合物,分别鉴定为α-tetrahydrobisabolen-2,5,6-triol(1)、10,11-dihydroxynero-lidol(2)、3,5,4'-三甲氧基-4-羟基-联苯(3,5,4'-trimethoxy-4-hydroxyl-biphenyl,3)、(+)-7R,8S-5-methoxy dihydrodehydroconiferyl alcohol(4)、5,4'-二甲氧基-联苯-4-羟基-3-O-β-D-葡萄糖苷(5,4'-dimethoxy-biphenyl-4-ol-3-O-β-D-glucoside,5)、槲皮素(quercetin,6)、牡荆素(vitexin,7)。结论化合物1为新天然产物,化合物2～4为首次从山楂属植物中分离得到。

Isolation and identification of constituents from leaves of Crataegus pinnatifida Bge.

Objective To study the chemical constituents of the leaves of Crataegus pinnatifida Bge..Methods Seven compounds were isolated by chromatography on silica gel column,Sephadex LH-20,ODS chromatography column and HPLC,their structures were confirmed on the basis of physico-chemical constants and spectroscopic analysis.Results Seven compounds were isolated from 80% ethanol-water extraction and their structures were identified as α-tetrahydrobisabolen-2,5,6-triol(1),10,11-dihydroxynerolidol(2),3,5,4′-trimethoxy-4-hydroxyl-biphenyl(3),(+)-7R,8S-5-methoxy dihydrodehydroconiferyl alcohol(4),5,4′-dimethoxy-biphenyl-4-ol-3-O-β-D-glucoside(5),quercetin(6),vitexin(7).Conclusions Compound 1 is a new natural product and compounds 2-4 are isolated from Crataegus genus for the first time.